Protein Info for MPMX19_06292 in Azospirillum sp. SherDot2

Annotation: Protein-glutamate methylesterase/protein-glutamine glutaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1051 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF01339: CheB_methylest" amino acids 10 to 186 (177 residues), 166.7 bits, see alignment E=1.6e-52 PF03705: CheR_N" amino acids 218 to 264 (47 residues), 46.1 bits, see alignment (E = 1.1e-15) PF01739: CheR" amino acids 279 to 466 (188 residues), 165 bits, see alignment E=5.2e-52 PF13596: PAS_10" amino acids 729 to 835 (107 residues), 85.3 bits, see alignment E=1.5e-27 PF08448: PAS_4" amino acids 734 to 838 (105 residues), 30.2 bits, see alignment E=1.6e-10 PF07536: HWE_HK" amino acids 850 to 929 (80 residues), 67.8 bits, see alignment 3.9e-22

Best Hits

KEGG orthology group: K13924, two-component system, chemotaxis family, CheB/CheR fusion protein [EC: 2.1.1.80 3.1.1.61] (inferred from 90% identity to azl:AZL_e00490)

Predicted SEED Role

"Chemotaxis protein methyltransferase CheR (EC 2.1.1.80)" in subsystem Bacterial Chemotaxis (EC 2.1.1.80)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.80, 3.1.1.61

Use Curated BLAST to search for 2.1.1.80 or 3.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1051 amino acids)

>MPMX19_06292 Protein-glutamate methylesterase/protein-glutamine glutaminase (Azospirillum sp. SherDot2)
MSDKASFPIVGIGASAGGVEALEGLFRALPADSGMAFVVVTHLAPQHESLLADIIGRCTS
TPARQAVDGDLVEPDRIYTLQPGTLLTIESGRLRVRPENAAHRERNPIDIFLSSLAGDCG
ERAVGIILSGSGSDGTLGVKAIKERGGFTIAQGHDGIHRHDGMPNSAIATGVVDLVAPVE
TMAATLVDYTRTATPLGDLTSEKEVREDARLEAARRSITTILRNQVGHDFRGYKHNTFLR
RVQRRMQALRLTDIDSYVGYLREDRTEAAALFRDLLIGVTTFFRDGETFQALEELVIPHL
FAGKSTGDAVRVWVPGCATGEEVYSIAMLLSEHAETLHSVPKIQVFATDIDEHALEVART
GRYPASLVRDVPADRLKRHFIEDGTSYVVRKEVRDLCIFSAHSLVRDPPFSRIDLISCRN
LLIYLGVELQDQVIPMFHYALRPSSYLFLGVAETIGQHQALFVPIDKKHRLFRRQDTASV
TIPFALAEHGGRSVRGGAETPRSIQSNAANLRGHVEARVLERFAPAHIVVNQNGEVVYYS
ANTGKYLQAPPGAPNRQLLAMARKGLRLDLRGALHEAMQTHKPVTRKHLAVEVDDRVQVL
DLTIEPFDREGEAFFLVLFSDVGRPSPPEEIYGKESQWGQDPVVVEQLERELRDTKERLQ
STIEEYETSLEELKSANEELISVNEELQSTNEELETAKEETQSVNEELHAVNIELNSKVE
ELDQSNNDLQNLFNSTQIGIVFLDRCLLIRSFTPAVTRIFNLIASDRGRPLTDIVSHLDQ
LDLDHDLGQALAGTEPVEKAVRSRDGTKHYIMRILPYRNARNDLEGLLLTFVDVTSLKEA
EEQQRQLIGELDHRVKNMLAVVIALAQQTLKTIAKAPECTAFLGRLQAVASTYDLLTRDR
WGNIPLNDIVLNEAKPYAGSEGRFVVEGPAVQLRPKAALALGMVIHELVTNAVKYGALSV
AEGRVVVTWEIGEKDADAALLVHWRETDGPVLSPPSHHGFGSKLITQQIRYELRGDATVD
YRPTGLEATFTVPIAQLRSDDEAPDFPESAR