Protein Info for MPMX19_06032 in Azospirillum sp. SherDot2

Annotation: Phosphate-binding protein PstS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 358 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details PF12849: PBP_like_2" amino acids 32 to 314 (283 residues), 146.7 bits, see alignment E=1.2e-46 TIGR00975: phosphate ABC transporter, phosphate-binding protein PstS" amino acids 35 to 342 (308 residues), 378.6 bits, see alignment E=1.1e-117

Best Hits

Swiss-Prot: 60% identical to PSTS_RHILO: Phosphate-binding protein PstS (pstS) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)

KEGG orthology group: K02040, phosphate transport system substrate-binding protein (inferred from 97% identity to azl:AZL_e02450)

MetaCyc: 52% identical to phosphate ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-27-RXN [EC: 7.3.2.1]; 7.3.2.1 [EC: 7.3.2.1]

Predicted SEED Role

"Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (TC 3.A.1.7.1)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (358 amino acids)

>MPMX19_06032 Phosphate-binding protein PstS (Azospirillum sp. SherDot2)
MKTMTSAFVRCAAFGALAVLSVSVAPISVASAADISGAGATFPYPIYAKWADAYKKETGT
GLNYQSIGSGGGIKQIKAKTVTFGASDMPLKPEELEQAGLIQFPMIMGGVVPVVNLKGIK
AGEVKLSGTVLANIYMGEITKWNDAQIKALNPNVNLPNTAIAPVYRSDGSGTNFLFTDYL
SKTSPKFKTQIGANTSVQWPAGIGAKGNEGVANMVKQTDGSIGYVEYAYAKQNNITHLDL
QNKDGKTVSPKIEAFQAAAANADWANARGYYVLLTDEPGADSWPITGASFILMYKAPQDV
ASSAEALKFFDWAYKNGGKMATDLDYVPMPEAVVSLVQKTWSQTIQADGKPVWTASAK