Protein Info for MPMX19_05884 in Azospirillum sp. SherDot2

Annotation: Multidrug efflux pump subunit AcrB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1050 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details amino acids 340 to 359 (20 residues), see Phobius details amino acids 366 to 388 (23 residues), see Phobius details amino acids 393 to 413 (21 residues), see Phobius details amino acids 438 to 460 (23 residues), see Phobius details amino acids 470 to 494 (25 residues), see Phobius details amino acids 540 to 559 (20 residues), see Phobius details amino acids 875 to 893 (19 residues), see Phobius details amino acids 900 to 920 (21 residues), see Phobius details amino acids 927 to 953 (27 residues), see Phobius details amino acids 975 to 995 (21 residues), see Phobius details amino acids 1005 to 1033 (29 residues), see Phobius details PF00873: ACR_tran" amino acids 1 to 1029 (1029 residues), 1336.3 bits, see alignment E=0 TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1037 (1037 residues), 1739 bits, see alignment E=0 PF03176: MMPL" amino acids 296 to 496 (201 residues), 24.5 bits, see alignment E=1.9e-09 PF02355: SecD_SecF" amino acids 339 to 495 (157 residues), 21 bits, see alignment E=3e-08 amino acids 879 to 1018 (140 residues), 25.5 bits, see alignment E=1.2e-09

Best Hits

Swiss-Prot: 68% identical to ACRB_ECOLI: Multidrug efflux pump subunit AcrB (acrB) from Escherichia coli (strain K12)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 66% identity to abo:ABO_0964)

MetaCyc: 61% identical to multidrug efflux pump RND permease AcrD (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-1551; TRANS-RXN-1552; TRANS-RXN-360

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1050 amino acids)

>MPMX19_05884 Multidrug efflux pump subunit AcrB (Azospirillum sp. SherDot2)
MSKFFIDRPVFAWVIAIVIMMAGALAILRLPVEQYPKIAPPTVSITASYPGASAKTLEDT
VTQVIEQKMTGLDHFRYMSSNSDSSGNVTITLTFEPEANPDIAQVQVQNKLQLATPLLPQ
EVQQRGIQVSKAGNDFLLVVGFVSSDGRLSQGDIADYVTSNLQDTLSRVEGVGDITVFGP
QHAMRIWLDPDALNNFALTTTDVSNAIKTQNAQVSAGQLGGAPAVQGQQINATISAQSRL
QTPEQFGAILLRVNPDGSQVRLRDVARIEIGSETYEINAAYNGKPAAGVGIKLATGANAL
NTAAAVKARVAELQPFFPAGIEVIYPYDTTPFVQLSIHEVIKTLFEAIVLVFVVMYLFLQ
NFRATLIPTIAVPVVLLGTFGVMSAFGFSINVLTMFGMVLAIGLLVDDAIVVVENVERVM
GEEGLPPREATRKSMDQITGALVGIALVLSAVFVPMAFFGGSAGAIYRQFSLTIVSAMAL
SVLVALVLTPALCATLLKPVANHGEGHGHARGGFFGLFNRGFDAGSRVYVRGVRGAVNRL
GRYFIAYALILGGLGYLFVQMPSSFLPTEDRGQLFVLWSAPPNASSQRTAETAKAVTAYM
LEKEKDSVEGLFTIVGFNFSGRGQNAGMAFVRLKDWSERTQASQKPQAIAGRVMGAMSKL
KDAVLFSFLPPSVPGLGNATGFDLQLVDRGGLGHDRLMQARNQLLGAAAQNPKLVGVRPN
GLEDTPQFKLDIDREKASALGLSLSDINTTLSAAWGSSYVNDFIDQGRVKKVYLQADAPY
RMQPSDIDRWYVRNAGGQMVPFSAFTTAQWIYGSPKLERYNGSSSVNIQGSAAPGISSGE
AMTEMETLMQKLPPGIGYEWTGLSYEERLSGSQAPALYALSMIIVFLCLAALYESWSVPV
SVIMVVPLGVIGAVIAATLRGLPSDVYFQVGLLTTIGLSAKNAILIVEFAKALYDQGMEL
KEAAAEACRQRLRPIIMTSMAFILGVLPLAISTGAGSESQNAIGVGVMGGMISATVLAVL
FVPVFFVAVYRLFVRRRPGHGAMPQPAAGD