Protein Info for MPMX19_05836 in Azospirillum sp. SherDot2

Annotation: Alkyl hydroperoxide reductase subunit F

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 596 TIGR03140: alkyl hydroperoxide reductase subunit F" amino acids 66 to 581 (516 residues), 796.4 bits, see alignment E=5e-244 PF13192: Thioredoxin_3" amino acids 189 to 257 (69 residues), 33.6 bits, see alignment E=1.2e-11 PF07992: Pyr_redox_2" amino acids 278 to 568 (291 residues), 168.5 bits, see alignment E=8e-53 PF13738: Pyr_redox_3" amino acids 323 to 551 (229 residues), 48.2 bits, see alignment E=3.2e-16 PF00070: Pyr_redox" amino acids 421 to 491 (71 residues), 50.6 bits, see alignment E=8.1e-17

Best Hits

Swiss-Prot: 75% identical to AHPF_PSEPU: Alkyl hydroperoxide reductase subunit F (ahpF) from Pseudomonas putida

KEGG orthology group: K03387, alkyl hydroperoxide reductase subunit F [EC: 1.6.4.-] (inferred from 94% identity to azl:AZL_d00900)

MetaCyc: 64% identical to alkyl hydroperoxide reductase, AhpF component (Escherichia coli K-12 substr. MG1655)
RXN-8506 [EC: 1.5.1.37]; R4-RXN [EC: 1.5.1.37, 1.11.1.26]

Predicted SEED Role

"Alkyl hydroperoxide reductase protein F (EC 1.6.4.-)" in subsystem Thioredoxin-disulfide reductase (EC 1.6.4.-)

Isozymes

Compare fitness of predicted isozymes for: 1.6.4.-

Use Curated BLAST to search for 1.11.1.26 or 1.5.1.37 or 1.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (596 amino acids)

>MPMX19_05836 Alkyl hydroperoxide reductase subunit F (Azospirillum sp. SherDot2)
MSQTFQNLDPRLRGEDDDVSNSSLFQNQTARPTGSSCSGHNFLRPDRRDYAQAIRPGHET
LGTKAMLDANLKTQLKAYLERITHPITLVASLDDGAKSQEMLGLLEDIASLSGKITLDRS
GADARRPSFAIKRDGSDIGVAFAGLPMGHEFNSLVLALLQVGGHPSKESEELLEQIRTLD
GDFRFETYFSLSCQNCPDVVQALNLMSALNPRIKHVAIDGALFQQEVEQRQVMAVPTVFL
NGEPFAQGRMDVAQIVAKLDTGAVARAAEKIKAKAPFEVLVIGGGPAGAAAAIYAARKGI
RTGVAAERFGGQVLDTMAIENFISVSHTEGPKLAAALEQHVKDYEVDVMNLQTATALVPA
TQEGGLIEVKLASGASLKSRTVVLSTGARWRSMNVPGEAEYRNKGVAYCPHCDGPLFKGK
RVAVIGGGNSGVEAAIDLAGIVSHVTLIEFDSQLRADAVLQRKLHSLPNVTVLLSAQTTE
VHGDGNRVVGLSYKERTGGELKRVDLEGIFVQIGLVPNTEWLKGTLALTPRGEIEVDNRG
QTSLPGVFAAGDATTVPFKQIVIAMGEGAKAGLAAFDHLIRTVPAEEPARETIAAE