Protein Info for MPMX19_05807 in Azospirillum sp. SherDot2

Annotation: N-carbamoyl-L-amino-acid hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 422 TIGR01879: amidase, hydantoinase/carbamoylase family" amino acids 13 to 409 (397 residues), 420.4 bits, see alignment E=3.6e-130 PF04389: Peptidase_M28" amino acids 66 to 133 (68 residues), 24.5 bits, see alignment E=3.2e-09 PF01546: Peptidase_M20" amino acids 83 to 408 (326 residues), 90.4 bits, see alignment E=2.4e-29 PF07687: M20_dimer" amino acids 215 to 313 (99 residues), 23.3 bits, see alignment E=7.8e-09

Best Hits

Swiss-Prot: 53% identical to HYUC_RHIML: N-carbamoyl-L-amino-acid hydrolase (hyuC) from Rhizobium meliloti

KEGG orthology group: K06016, N-carbamoyl-L-amino-acid hydrolase [EC: 3.5.1.87] (inferred from 95% identity to azl:AZL_d01280)

MetaCyc: 53% identical to N-carbamoyl-L-amino-acid hydrolase subunit (Sinorhizobium meliloti CECT4114)
N-carbamoyl-L-amino-acid hydrolase. [EC: 3.5.1.87]

Predicted SEED Role

"Beta-ureidopropionase (EC 3.5.1.6)" in subsystem Hydantoin metabolism or L-2-amino-thiazoline-4-carboxylic acid-Lcysteine conversion or Pyrimidine utilization (EC 3.5.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.6, 3.5.1.87

Use Curated BLAST to search for 3.5.1.6 or 3.5.1.87

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (422 amino acids)

>MPMX19_05807 N-carbamoyl-L-amino-acid hydrolase (Azospirillum sp. SherDot2)
MSTPQNVAVNGSRLWQSLMDMAQIGGTPKGGVCRLALTDLDKQGRDLFVRWCEEAGCTIS
VDRMGNIFARRPGRDDSLPPVIMGSHLDSQPTGGKYDGVYGVLAGLEVVRSLNDMNYVTE
APVEVAVWTNEEGSRFAPAMVSSGVFAGVFDLDYGLSRADLDGKTMGEELARIGYAGDQE
VGGRKVGAYFEAHIEQGPILEIEGKTIGVVTDCQGQRWYEITFTGQEAHAGPTPMVRRKD
ALLGAARVVDAVNRIGMKYGPLACATVGLMQIHPNSRNVIPGRVFFTVDFRHPDDGILKA
MDGELRAEVERIAGEIGLETDFQQIWYYAPIRFDETCVAAVRKGVERTGHSFRDIVSGAG
HDACYLSKVAPTAMVFIPCIDGISHNEVEETTPEWSTAGCEVLLHAVLDRANAMAEQVKA
PA