Protein Info for MPMX19_05593 in Azospirillum sp. SherDot2

Annotation: Pyrrolidone-carboxylate peptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 215 PF01470: Peptidase_C15" amino acids 4 to 200 (197 residues), 244.6 bits, see alignment E=5.2e-77 TIGR00504: pyroglutamyl-peptidase I" amino acids 4 to 214 (211 residues), 255 bits, see alignment E=3.5e-80

Best Hits

Swiss-Prot: 59% identical to PCP_CUPTR: Pyrrolidone-carboxylate peptidase (pcp) from Cupriavidus taiwanensis (strain DSM 17343 / BCRC 17206 / CIP 107171 / LMG 19424 / R1)

KEGG orthology group: K01304, pyroglutamyl-peptidase [EC: 3.4.19.3] (inferred from 91% identity to azl:AZL_d03120)

MetaCyc: 40% identical to pyroglutamyl-peptidase monomer (Staphylococcus aureus)
Pyroglutamyl-peptidase I. [EC: 3.4.19.3]

Predicted SEED Role

"Pyrrolidone-carboxylate peptidase (EC 3.4.19.3)" in subsystem ZZ gjo need homes (EC 3.4.19.3)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.4.19.3

Use Curated BLAST to search for 3.4.19.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (215 amino acids)

>MPMX19_05593 Pyrrolidone-carboxylate peptidase (Azospirillum sp. SherDot2)
MPTVLLTGFEPFGGEPVNPSWEAVRQLDGWDCRGHRVAARLMPCVFGAALDALERAVAET
DPVALLAVGQAQSRAELSLERVAVNLDDARIPDNAGNRPVDAPVVADGPAAYFASLPVKA
MAAALRDAGIPAGLSHSAGSFVCNHLFYGACHLRAVRRPGLRVGFLHIPLAPEQAVRHPG
MPSMAIDRVVAGLRIAVDTLLATGEDRHEAAGTLE