Protein Info for MPMX19_05415 in Azospirillum sp. SherDot2

Annotation: Homogentisate 1,2-dioxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 434 TIGR01015: homogentisate 1,2-dioxygenase" amino acids 6 to 429 (424 residues), 666.4 bits, see alignment E=7.8e-205 PF20510: HgmA_N" amino acids 7 to 275 (269 residues), 416.5 bits, see alignment E=4.8e-129 PF04209: HgmA_C" amino acids 276 to 429 (154 residues), 242.5 bits, see alignment E=1.6e-76

Best Hits

Swiss-Prot: 79% identical to HGD_RALSO: Homogentisate 1,2-dioxygenase (hmgA) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K00451, homogentisate 1,2-dioxygenase [EC: 1.13.11.5] (inferred from 94% identity to azl:AZL_d04870)

MetaCyc: 53% identical to Homogentisate 1,2-dioxygenase (Homo sapiens)
Homogentisate 1,2-dioxygenase. [EC: 1.13.11.5]

Predicted SEED Role

"Homogentisate 1,2-dioxygenase (EC 1.13.11.5)" in subsystem Homogentisate pathway of aromatic compound degradation (EC 1.13.11.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.13.11.5

Use Curated BLAST to search for 1.13.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (434 amino acids)

>MPMX19_05415 Homogentisate 1,2-dioxygenase (Azospirillum sp. SherDot2)
MTSSAFTYRSGFGNEFATEALPGALPVGRNSPQRPPYGLYAEQISGTAFTAPRAHNRRSW
LYRIRPAVVHEPFRPLESGRLTSRFDEVPAPPTQLRWNPPPMPDAPTDFIAGLATMGGNG
GPQAQTGCGIHLYAANRSMEGRFFYDADGELLIVPQQGRLRLFTELGVLEVEPQEIALIP
RGIRFRVELPDGTARGYVCENFGAPFRLPDLGPIGSNGLANPRDFLTPDAWYEDVDGDFE
LVAKFDGALWTARIDHSPLDVVAWHGNHAPCKYDLRRFNAIGSIGFDHPDPSIFLVLHSP
SDTPGVDSIDFVIFPPRWLVQEDTFRPPWFHRNVASEFMGLIHGVYDAKEEGFLPGGASL
HNCMSGHGPDAETFAKATAADTSRPQRVADTMAFMVETRAVIRPTRHALETAELQHDYHR
CWQGLAKRFDPNRP