Protein Info for MPMX19_05319 in Azospirillum sp. SherDot2
Annotation: Cystathionine gamma-synthase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 41% identical to CGL_RAT: Cystathionine gamma-lyase (Cth) from Rattus norvegicus
KEGG orthology group: K01739, cystathionine gamma-synthase [EC: 2.5.1.48] (inferred from 94% identity to azl:AZL_c02520)MetaCyc: 41% identical to cystathionine gamma-lyase subunit (Rattus norvegicus)
Cystathionine gamma-lyase. [EC: 4.4.1.1]; 4.4.1.1 [EC: 4.4.1.1]
Predicted SEED Role
"Cystathionine gamma-lyase (EC 4.4.1.1)" in subsystem Cysteine Biosynthesis or Glycine and Serine Utilization or Methionine Biosynthesis or Methionine Degradation (EC 4.4.1.1)
MetaCyc Pathways
- aspartate superpathway (23/25 steps found)
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- superpathway of L-isoleucine biosynthesis I (13/13 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
- superpathway of L-threonine metabolism (15/18 steps found)
- L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
- superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (9/10 steps found)
- L-threonine degradation I (6/6 steps found)
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (12/15 steps found)
- glycine betaine degradation III (6/7 steps found)
- D-serine degradation (3/3 steps found)
- L-cysteine degradation II (3/3 steps found)
- L-methionine degradation II (3/3 steps found)
- L-serine degradation (3/3 steps found)
- superpathway of L-cysteine biosynthesis (fungi) (5/6 steps found)
- superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
- superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
- glycine betaine degradation I (6/8 steps found)
- superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
- homocysteine and cysteine interconversion (3/4 steps found)
- L-tryptophan degradation II (via pyruvate) (2/3 steps found)
- purine nucleobases degradation II (anaerobic) (17/24 steps found)
- L-methionine biosynthesis II (4/6 steps found)
- L-cysteine biosynthesis III (from L-homocysteine) (1/2 steps found)
- L-methionine biosynthesis I (3/5 steps found)
- superpathway of L-cysteine biosynthesis (mammalian) (3/5 steps found)
- felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis (2/5 steps found)
- L-mimosine degradation (4/8 steps found)
- L-cysteine biosynthesis VI (reverse transsulfuration) (3/7 steps found)
- superpathway of L-methionine salvage and degradation (9/16 steps found)
- glutathione-mediated detoxification I (3/8 steps found)
- hydrogen sulfide biosynthesis II (mammalian) (1/6 steps found)
- hypoglycin biosynthesis (4/14 steps found)
KEGG Metabolic Maps
- Biosynthesis of plant hormones
- Cysteine metabolism
- Glycine, serine and threonine metabolism
- Methionine metabolism
- Nitrogen metabolism
- Selenoamino acid metabolism
- Sulfur metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.5.1.48, 4.4.1.1
Use Curated BLAST to search for 2.5.1.48 or 4.4.1.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (381 amino acids)
>MPMX19_05319 Cystathionine gamma-synthase (Azospirillum sp. SherDot2) MTDRPHRPETIAAGALGFEDAASGAVIPPIHPSTTYVRDPDNGYGRGRVYARADNPTFDI PARTITELEKGTDTLLFASGMAAATAVFQALDPGDHVIVPDVMYWAFRNWVHGPATRWGL KVEGVDTSDTDAVRAALRPGQTRLVWVETPANPLWTVTDIAAVAELAHEAGAWLAVDSTV ATPVLTRPLELGADIVMHSATKYLNGHSDVVAGTLTAARKDEFWARIKAVQTQQGGILGS FEAWLLQRGLRTLFARVRWQSDAAMALAERLSAHPRVAEVLYPGLPGFPGHGIASRQMSG GYGGMLSVRVKGGTQAAIDTAARVEIWRRATSLGGVESLIEHRASIEGPSSPVPGDLLRL SVGLESPDDLYEDLSQALGEG