Protein Info for MPMX19_05285 in Azospirillum sp. SherDot2

Annotation: Carbamoyltransferase HypF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 735 TIGR00143: carbamoyltransferase HypF" amino acids 1 to 731 (731 residues), 739.8 bits, see alignment E=1.6e-226 PF00708: Acylphosphatase" amino acids 2 to 79 (78 residues), 59.9 bits, see alignment E=6.1e-20 PF07503: zf-HYPF" amino acids 98 to 130 (33 residues), 57.5 bits, see alignment (E = 2.3e-19) amino acids 148 to 179 (32 residues), 56.2 bits, see alignment (E = 5.9e-19) PF01300: Sua5_yciO_yrdC" amino acids 188 to 377 (190 residues), 145 bits, see alignment E=4.5e-46 PF17788: HypF_C" amino acids 392 to 486 (95 residues), 118 bits, see alignment E=6.4e-38 PF22521: HypF_C_2" amino acids 495 to 727 (233 residues), 229.2 bits, see alignment E=1.6e-71

Best Hits

Swiss-Prot: 56% identical to HYPF_AZOVI: Carbamoyltransferase HypF (hypF) from Azotobacter vinelandii

KEGG orthology group: K04656, hydrogenase maturation protein HypF (inferred from 85% identity to azl:AZL_c02020)

Predicted SEED Role

"[NiFe] hydrogenase metallocenter assembly protein HypF" in subsystem NiFe hydrogenase maturation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (735 amino acids)

>MPMX19_05285 Carbamoyltransferase HypF (Azospirillum sp. SherDot2)
MRGRVQGVGFRPHVHALAGRFSLTGWVLNDPQGVLIEVQGNGVGAFRAALSAEAPPLARI
DAVEFDAVPPVSGEDSFAIRHSLSAGAVATGIGPDATVCPACLAELFDPADRRHRYAFLN
CTHCGPRYTITRQLPYDRPQTAMAGFPLCPDCLAEYEDPANRRFHAQPTACPVCGPCLSH
PPEDIVAALRAGMIVAIKGLGGFHLACDATRADAVERLRRVKQRNGKPFALMVANTESAT
RHVRMDAGERALLEGVARPIVLLRRRDDGPELAPEIAPGLAWLGVMLPYTPLHYLLFHEA
AGRPGGTGWLDLPQDLTLVMTSANPGGEPLAIGNAEARERLAGIADLIVDHDRDIVIRAD
DSVVRRLAGAPAFLRRGRGHVPEPIRLAHPVAPVLAVGGHLKTTVCVTRGDEAFLSQHIG
DLDNAATLRFLEETVAHLLHILGVEPVAVAHDRHPDFHSTRFAEMSGLPTVPVQHHHAHV
AAVMAEHRVEGPAIGLALDGFGLGEDGGSWGGEMLLVDGLRSERLGSLAPLAQPGGDVAA
RQPWRMAAAALARMGRADEIASRFTAHGPAEGVRRMIEHGINAPLTSSCGRWFDAACGLL
GIRAVAGFEGEAPMALESLVRQIRVAEGAWRIDGSELDLTPLLERLLTVADAVEGAELFH
GTLAAALVDWTLPVLRAHGMGRVVLSGGCLMNALLAEELMAGFRSAGVEALLPRLAPAND
GGLSLGQAWAASFAL