Protein Info for MPMX19_04725 in Azospirillum sp. SherDot2

Annotation: Glycine betaine/choline transport system ATP-binding protein OusV

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 427 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details TIGR01186: glycine betaine/L-proline transport ATP binding subunit" amino acids 71 to 423 (353 residues), 429.6 bits, see alignment E=5.1e-133 PF00005: ABC_tran" amino acids 77 to 223 (147 residues), 125.1 bits, see alignment E=1.7e-40

Best Hits

Swiss-Prot: 56% identical to OUSV_DICD3: Glycine betaine/choline transport system ATP-binding protein OusV (ousV) from Dickeya dadantii (strain 3937)

KEGG orthology group: K02000, glycine betaine/proline transport system ATP-binding protein [EC: 3.6.3.32] (inferred from 94% identity to azl:AZL_c01830)

Predicted SEED Role

"L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (TC 3.A.1.12.1)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.32

Use Curated BLAST to search for 3.6.3.32

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (427 amino acids)

>MPMX19_04725 Glycine betaine/choline transport system ATP-binding protein OusV (Azospirillum sp. SherDot2)
MNTMMRSAVSVGGPASLGFPRGAARTGIGEDAAVRLEVRNVYKVFSADPKPALSMLRQGA
SKAEVLEKLNVNVGVLDASFQVRAGEIFVIMGLSGSGKSTMIRLLNRLIEPSSGEILLDG
KDLVSMSKADLIQARRRNMGMVFQSFALMPHLTALDNAAFGLEISGASKAERHKAAQQAL
EQVGLGTYAHRYPREMSGGMQQRVGLARALANDPTILLMDEAFSALDPLIRTEMQDELLR
LQREQQRTIVFISHDLDEAMRIGDRIAIMEGGRIVQVGTPYEILSRPADDYVRSFFRNVD
ASKVLRAGDVARFEATNTVIRLPGELAPALQQLRENDHNFGYVRTPEGHFEGIVSRATLE
RELNGPQPRFVNAFLPGVRTVNAAAPIHSLLALVADSEWPVPVVDDDDNFLGAISRALML
HTLVRGD