Protein Info for MPMX19_04721 in Azospirillum sp. SherDot2

Annotation: putative N-methylproline demethylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 681 PF00724: Oxidored_FMN" amino acids 8 to 343 (336 residues), 198.2 bits, see alignment E=9.3e-62 PF00070: Pyr_redox" amino acids 391 to 457 (67 residues), 25.5 bits, see alignment E=6e-09 PF03486: HI0933_like" amino acids 391 to 431 (41 residues), 24.5 bits, see alignment 4.6e-09 PF07992: Pyr_redox_2" amino acids 391 to 630 (240 residues), 50 bits, see alignment E=1.2e-16 PF00890: FAD_binding_2" amino acids 392 to 431 (40 residues), 21.8 bits, see alignment 4.1e-08 PF12831: FAD_oxidored" amino acids 392 to 442 (51 residues), 32.9 bits, see alignment 1.9e-11 PF13450: NAD_binding_8" amino acids 394 to 428 (35 residues), 28.8 bits, see alignment (E = 5e-10)

Best Hits

KEGG orthology group: None (inferred from 98% identity to azl:AZL_c01790)

MetaCyc: 52% identical to N,N-dimethylglycine/sarcosine dehydrogenase (ferredoxin) (Chromohalobacter salexigens)
RXN-22743 [EC: 1.5.7.3]; 1.5.7.3 [EC: 1.5.7.3]

Predicted SEED Role

"probable FMN oxidoreductase"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.7.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (681 amino acids)

>MPMX19_04721 putative N-methylproline demethylase (Azospirillum sp. SherDot2)
MSSPAEALLKPLQIKHLTIRNRVMSTSHAPGYGSEGKPKERYQLYHEEKAKGGIGLTMFG
GSSSVAVDSPAAPWNQISVADDSVIPFFREFSDRVHRHGAALMIQLTHMGRRTRWDTENW
LPTVSASAAREPAHRNTPKEMEDFDFKRIIAGYAAAARRCKEGGLDGFELSAAHGHLIDQ
FWSPSANRRSDRYGGSLENRMRFGIEVFEAIRAEVGDDYVIGMRMSGDELIDDGLSHEDC
IAIARTYAERGLIDFVNVLGGQARDHLAHAISLPNMSFPVAPFLHLASGIKREAGIPVFH
AQRVTDLATAARAVEEGHVDMVAMTRAHIADPHLVRKLMEGRLDDVRQCVGAGYCIDRIY
VGGDALCIQNAATGRERTMPHVIPKAERKRRVVVVGGGPGGLEAARVCAERGHSVVLLER
NAAPGGQIGIAAKATWREAMSGITRWLSQQVTKLGVDVRLGVEADRAAVEAENPDVVILA
TGGRPNRGDFKGADLAVSTWDILTGAVEPTGTVLMYDGTGQHHGASCAEFMAKRGALVEI
ATPDRMVAEEVGTTNQPIHLRELYKLNVVMTTNQQLVEIYPEGNRRVAVLRNEYTHEEEE
RLVDQIVVELGTLPNDGLYFDLKDLSLNRGETDLDSLIAGRPQPVQANGHGGYTLHRIGD
AVAGRNIHAAIYDAARLCKDI