Protein Info for MPMX19_04621 in Azospirillum sp. SherDot2

Annotation: NAD-specific glutamate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF21075: GDH_ACT1
  • amino acids 32 to 179 (148 residues), 184.7 bits, E=3.2e-58
• PF21073: GDH_HM1
  • amino acids 348 to 407 (60 residues), 98.8 bits, see alignment (E = 5.2e-32)
• PF21076: GDH_ACT2
  • amino acids 412 to 499 (88 residues), 112 bits, 6.6e-36
• PF21079: GDH_HM2
  • amino acids 506 to 549 (44 residues), 71.6 bits, see alignment (E = 1.7e-23)
• PF21077: GDH_ACT3
  • amino acids 556 to 635 (80 residues), 89.3 bits, 4.4e-29
• PF21078: GDH_HM3
  • amino acids 654 to 719 (66 residues), 100.2 bits, see alignment (E = 2.3e-32)
• PF05088: Bac_GDH_CD
  • amino acids 731 to 1233 (503 residues), 868.2 bits, E=6.4e-265
• PF21074: GDH_C
  • amino acids 1279 to 1612 (334 residues), 418.9 bits, E=6.6e-129

Predicted SEED Role

"NAD-specific glutamate dehydrogenase (EC 1.4.1.2), large form" in subsystem Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.2)

MetaCyc Pathways

• L-glutamate degradation I (1/1 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (7/10 steps found)
• L-glutamate degradation XI (reductive Stickland reaction) (4/7 steps found)
• L-alanine degradation II (to D-lactate) (1/3 steps found)
• ethene biosynthesis IV (engineered) (1/3 steps found)
• methylaspartate cycle (12/19 steps found)
• 4-aminobutanoate degradation V (3/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.1.2

Protein Sequence (1617 amino acids)

>MPMX19_04621 NAD-specific glutamate dehydrogenase (Azospirillum sp. SherDot2)
MRTATKDLAGDLRQQLAELVRSRVPNARAERFVKRFYANVPPDDLLRGTPEQLYGAALAM
WQWGQQRNAGTAKVRVYAPRLDEHGWQAERSVVEIVNDDMPFLVDSVTAELNRQGVTVHL
IIHPVTRVVRDADGRIVELLEPEQNPGGEEADGAHDESFMHCSIDPLSDPDAQARLREGI
ERVLADVRAAVEDWAPMRERVRAARDDIAHAPDAEESQEAADFLSWIDDGNMTLLGSRLY
TATIGGAGQEPWLELVEGSGLGVLRAPEITVFDEHGHAAVLPEEIRAFLHQPRALLVTKG
TRQATVHRSVPLDAILVKRFDGEGKVVGVTLAVGLFTSVAYNRSPREIPFLRRKVARVMG
RAGFDPSGHDGKALLNILETYPRDELFQTPSDELFETAVGILYLQERQRLALFVRRDPFE
RFVSALVFVPRDRYDTALRRKIQSVLESAFHGTCTSYFTQLSDSALARLHLMVKTEPGKL
PPVDIGEIEARLVQVSRSWADRLRDALVEAHGEETGNARLRRYADAFPAGYRETFSAETA
VHDIDRIERALTEQRLGIVLFHPLEADGDELHVKIYHQGRPVPLSDVLPMLEHMDLKVIT
EQPYEVRPAGGSTPAQSVWIHDFAARTQNGMPVDCVKVKQTFQEAFADIWDGRMEDDGFN
RLVLRAGLAGREVTILRAYAKYLKQARFAYAQDTIETTLAAHPQTARLLARLFAARFDPK
NLLDEAPILEQIETALDAVTNLDDDRILRRFVNLIRATLRTNAYQTGPDGAPKPHLSFKL
DSGSIEELPLPRPWVEVFVYSPRMEGVHLRGGKVARGGIRWSDRREDFRTEILGLMKAQM
VKNTVIVPVGSKGGFVVKRPPPVSAGREAALAEGIECYKTLMRGLLDITDNLSADGSVVP
ATDVVRHDADDPYLVVAADKGTATFSDIANGVSIDHGFWLGDAFASGGSAGYDHKVMGIT
ARGAWESVKRHFREMGTDIQTTDFTVVGVGDMSGDVFGNGMLLSRHIRLLAAFDHRHIFL
DPDPDAAASWEERNRLFALPRSSWADYDRSRLSKGAMIVERSAKTVELTAEVRACFGIEQ
PHLSPVELMRRLLTVEVDLLWFGGIGTYIKASSETNAEAGDKANDALRIDGGQIRAKVIG
EGANLGVTQRGRIEAAQKGRDGKGVRLNTDAIDNSAGVDTSDHEVNIKILLGDVMARGDM
TLKQRDTLLAAMTDEVAGLVLADNYRQSQALTIAEAQGAGLLEAQLRFIRNLERNGRLNR
AIEYLPTDEELAQRMAERRGLTRPELAVLLAYAKITLYDDLLASELPDDPATVEDLLRYF
PQPLRDGQGEAISRHRLRREIIATAVTNSLVNRVGPTFVRDMVEKTGLGPADVARAYAIA
RDVFQLRPLWDAIDALDTVVPAALQTALMLETIQLLDRAVAWFLAHSPHPLDLGRESAAF
RPGVEALAAGLDRFLDAEESSRLATRIADAMAQGVPEDLARRIAALPVLAAAPDLVRIAE
RTGRAVEGVASVYFGLGRRFGLEWLRDRAAGAKVDNHWQRQAVAAIVDDLFAHQSALTVR
VLESEGDEAAAVDGWIANRNLVVERVEQLLAELRAQPAVDLAMLAVANRQLRGLIAG