Protein Info for MPMX19_04365 in Azospirillum sp. SherDot2

Annotation: Thymidine phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 439 TIGR02643: thymidine phosphorylase" amino acids 1 to 437 (437 residues), 651.4 bits, see alignment E=7.1e-200 TIGR02644: pyrimidine-nucleoside phosphorylase" amino acids 4 to 422 (419 residues), 503.7 bits, see alignment E=3.7e-155 PF02885: Glycos_trans_3N" amino acids 4 to 66 (63 residues), 74.7 bits, see alignment E=6.1e-25 PF00591: Glycos_transf_3" amino acids 79 to 308 (230 residues), 142.8 bits, see alignment E=2.3e-45 PF07831: PYNP_C" amino acids 349 to 422 (74 residues), 61.6 bits, see alignment E=6.9e-21

Best Hits

Swiss-Prot: 58% identical to TYPH_SHEHH: Thymidine phosphorylase (deoA) from Shewanella halifaxensis (strain HAW-EB4)

KEGG orthology group: K00758, thymidine phosphorylase [EC: 2.4.2.4] (inferred from 95% identity to azl:AZL_a04140)

MetaCyc: 56% identical to thymidine phosphorylase (Escherichia coli K-12 substr. MG1655)
Pyrimidine-nucleoside phosphorylase. [EC: 2.4.2.2, 2.4.2.3]; Thymidine phosphorylase. [EC: 2.4.2.2, 2.4.2.3, 2.4.2.4]

Predicted SEED Role

"Thymidine phosphorylase (EC 2.4.2.4)" in subsystem Deoxyribose and Deoxynucleoside Catabolism (EC 2.4.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.4

Use Curated BLAST to search for 2.4.2.2 or 2.4.2.3 or 2.4.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (439 amino acids)

>MPMX19_04365 Thymidine phosphorylase (Azospirillum sp. SherDot2)
MLPQEIVRAKRDGQPLDAATIQDFVRGITDGRVSEAQASAFAMAVFFRGMSLDERVALTR
AMRDSGTVMEWKSLDLPGPVIDKHSTGGVGDKVSLILAPMLAACGGFVPMVSGRGLGHTG
GTLDKFESIPGYRAKPDNELLRRVVKEVGCAVIGATDDIAPADKRLYAIRDVTATVESLD
LITASILSKKLAAGLDALVMDVKFGSGAFMQRFEDAEALAESIVTVSKGAGLPAVALLTD
MNEVLGRSAGNALEMRECLAILRGEPAEPRLYEVTAALAAELLALAGLAPDAAAGRVLAD
RSIASGEAAERFARMVKALGGPADLLEQPDRHLESAPIVRPLFAERAGFVGAIDTRGVGM
AVVALGGGRTRTTDAIDFAVGFDGVAGLGDAVGPGDRPLAILHARSEAQAGEAERRLRAS
VTLTDTAPVRGPLIAKRLG