Protein Info for MPMX19_04300 in Azospirillum sp. SherDot2

Annotation: Quinoprotein alcohol dehydrogenase (cytochrome c)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 587 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 32 to 557 (526 residues), 802.8 bits, see alignment E=7.2e-246 PF13570: PQQ_3" amino acids 74 to 114 (41 residues), 20.9 bits, see alignment 5.9e-08 amino acids 481 to 521 (41 residues), 31.7 bits, see alignment 2.3e-11 PF13360: PQQ_2" amino acids 74 to 243 (170 residues), 64.5 bits, see alignment E=1.9e-21 amino acids 472 to 539 (68 residues), 35.5 bits, see alignment E=1.3e-12 PF01011: PQQ" amino acids 96 to 122 (27 residues), 25.8 bits, see alignment (E = 9.9e-10) amino acids 145 to 176 (32 residues), 23.3 bits, see alignment (E = 6.1e-09) amino acids 503 to 539 (37 residues), 38.4 bits, see alignment 1e-13

Best Hits

Swiss-Prot: 58% identical to QEDH_PSEPU: Quinoprotein alcohol dehydrogenase (cytochrome c) (qedA) from Pseudomonas putida

KEGG orthology group: K00114, alcohol dehydrogenase (cytochrome c) [EC: 1.1.2.8] (inferred from 97% identity to azl:AZL_a05260)

MetaCyc: 57% identical to quinoprotein alcohol dehydrogenase (Pseudomonas putida U)
1.1.2.-

Predicted SEED Role

"Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8)" (EC 1.1.99.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.8, 1.1.99.8

Use Curated BLAST to search for 1.1.2.8 or 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (587 amino acids)

>MPMX19_04300 Quinoprotein alcohol dehydrogenase (cytochrome c) (Azospirillum sp. SherDot2)
MKRLVTSLALAASLTAFSATGVLAADAGGPSSDDLLKSATNTEAVLTYGMGPQAQRFSPL
TDLTADTVKKLVPVWSFSFGGEKQRGQEAQPIIYDGTIYVTGSYSRLYAVDARTGHKKWE
YNHRLPDGIMPCCDVVNRGAAIYKDKIYFATLDARLVALNRETGKVVWNKKLQEYKEGYS
NTAAPLIIDGKIITGNSGGEFGVIGMVEARDAETGELVWQRPTIEGNMGTLNGKDSTVTG
KTNASWPGDMYKTGGGATWLGGTYDPETKTLFFGTGNPAPWNSHLRPGDNLYTSSTLAIN
PDNGEIKWHYQTTPHDGWDFDGVNEFVSFDLKKDGKVIKAGGKADRNGFFYVIDRTNGKL
INASPFVTKITWAKGIDIETGRPMYNDDNRPGAPATADAHDAKGKSVFAAPAFLGAKNWM
PMAYNPQTELFYVPANEWGMDIWNEPITYKKGAAYLGAGFTIKPLYDDYIGALRAVDPKT
GKIVWEYKNPAPLWGGVLTTAGNLVFTGTPEGYLKAFDAKSGQEVWKFQTGSGVVGSPVT
WKMDGEQYVAVVSGWGGAVPLWGGDVAKLVKDINQGGSLWVFKLPKA