Protein Info for MPMX19_04253 in Azospirillum sp. SherDot2

Annotation: Aerobic C4-dicarboxylate transport protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 443 transmembrane" amino acids 22 to 44 (23 residues), see Phobius details amino acids 64 to 83 (20 residues), see Phobius details amino acids 95 to 116 (22 residues), see Phobius details amino acids 165 to 184 (20 residues), see Phobius details amino acids 201 to 223 (23 residues), see Phobius details amino acids 241 to 264 (24 residues), see Phobius details amino acids 301 to 317 (17 residues), see Phobius details amino acids 323 to 341 (19 residues), see Phobius details amino acids 347 to 392 (46 residues), see Phobius details PF00375: SDF" amino acids 26 to 418 (393 residues), 385.5 bits, see alignment E=1.4e-119

Best Hits

Swiss-Prot: 64% identical to DCTA_PECCP: C4-dicarboxylate transport protein (dctA) from Pectobacterium carotovorum subsp. carotovorum (strain PC1)

KEGG orthology group: K11103, aerobic C4-dicarboxylate transport protein (inferred from 96% identity to azl:AZL_a05650)

MetaCyc: 62% identical to C4 dicarboxylate/orotate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-121; TRANS-RXN-121A; TRANS-RXN-121C; TRANS-RXN-122A; TRANS-RXN0-451; TRANS-RXN0-517; TRANS-RXN0-553

Predicted SEED Role

"Aerobic C4-dicarboxylate transporter for fumarate, L-malate, D-malate, succunate" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (443 amino acids)

>MPMX19_04253 Aerobic C4-dicarboxylate transport protein (Azospirillum sp. SherDot2)
MSADATASHRGAMNQGTVKKPFYTNLTFQVLTAISIGILLGHFYPSIAVEMKPLGDLFIK
MVKMVIGPIVFLTVVTGISSIGNLKKVGKVGGKALLYFEVVTTFALGIGLIVVNLVRPGA
GMNIVPGQLKADAVQKYATSAKEHGFMDFVVGIVPDNVVGAFVQGDMLQILFFAVVFGVA
LSAMGQKGKVVEDIFDKISHALFGVIGILMRVAPVGAFGAMSFTIGKYGISSLTALGQLM
ASVYLTMAIFIFVVLGSIAKAYGFSIWNFIRYIRQELLIVLGTSSSESVLPRMMEKMQKF
GCSKHVVGLVIPTGYSFNLDGTSIYLSMAAIFIAQAFNVDLTIWQQLYILVILMLTSKGA
AAVTGGGFVTLAATLSATGVLPIEGLALLLGVDRFMSEARALTNLIGNGVATVVVAKMEG
EFDESKAVAEYQQHFKEPALARL