Protein Info for MPMX19_04004 in Azospirillum sp. SherDot2

Annotation: Glutathione import ATP-binding protein GsiA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 637 PF00005: ABC_tran" amino acids 36 to 201 (166 residues), 103 bits, see alignment E=5.4e-33 amino acids 350 to 509 (160 residues), 119.1 bits, see alignment E=5.7e-38 PF08352: oligo_HPY" amino acids 252 to 284 (33 residues), 34.5 bits, see alignment (E = 5.5e-12) amino acids 560 to 606 (47 residues), 35.9 bits, see alignment 2e-12 TIGR01727: oligopeptide/dipeptide ABC transporter, ATP-binding protein, C-terminal domain" amino acids 558 to 608 (51 residues), 40.6 bits, see alignment 3.2e-14

Best Hits

Swiss-Prot: 59% identical to GSIA_ECO57: Glutathione import ATP-binding protein GsiA (gsiA) from Escherichia coli O157:H7

KEGG orthology group: K13892, glutathione transport system ATP-binding protein (inferred from 95% identity to azl:AZL_a08310)

MetaCyc: 61% identical to glutathione ABC transporter ATP binding subunit GsiA (Escherichia coli K-12 substr. MG1655)
RXN0-11 [EC: 7.4.2.10]

Predicted SEED Role

"Dipeptide transport ATP-binding protein DppD (TC 3.A.1.5.2)" in subsystem ABC transporter dipeptide (TC 3.A.1.5.2) (TC 3.A.1.5.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (637 amino acids)

>MPMX19_04004 Glutathione import ATP-binding protein GsiA (Azospirillum sp. SherDot2)
MSGAAAVKAVDTAPALLSVEGLSVAFDGDHGPVRVVDDVSFTVAPGETVAIVGESGSGKS
VTSLSLMRLVDYAGGRIVGGRIRFKGRDGRERDLATPPAEALTDMRGNDIAMVFQEPMTS
LNPVFTIGDQIAEAVMLHQGLDRKAARAEALRMLEKVRIPEPARLLDRHPHQLSGGMRQR
VMIAMALSCKPSLLIADEPTTALDVTIQAQILRLIRLLQDEMGMGVIFITHDMGVVAEVA
DRVVVMWRGRKVEEGPVERIFQAPQHPYTKALLSAVPKLGALRGQDLPVRFPLLVVDDAK
AEAAPPAPPPVQDTVRATDAPLLEVRDLTTRFDVRKGIFGSLKARVHAVEKVSFAVRPGE
TLALVGESGCGKSTTGRTIMGLQSATSGTIRFDGLDTATLDSNGQAKLKEKIQYIFQDPF
ASLNPRMTVGFSVAEPMLVHDYAPASRPGLSRRRAIEERVAELLTQVGLKPEHARRYPHE
FSGGQRQRICIARAIACDPKLIIADEAVAALDVSIQAQIVNLLMELQAKRGLSYLFISHD
MAVVERVSHRVAVMYLGQIVELGPRRAVFENPQHPYTRRLMAAVPIADPSRRTRDLRLLT
GEIPSPIRSIGDEPPVQPLVPVGEDHFVARHAVGGDF