Protein Info for MPMX19_03821 in Azospirillum sp. SherDot2

Annotation: Putative formate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 951 PF13510: Fer2_4" amino acids 19 to 94 (76 residues), 87.3 bits, see alignment E=2.3e-28 PF10588: NADH-G_4Fe-4S_3" amino acids 101 to 140 (40 residues), 60.3 bits, see alignment (E = 4.2e-20) PF12838: Fer4_7" amino acids 166 to 223 (58 residues), 32.1 bits, see alignment 5.1e-11 PF00037: Fer4" amino acids 208 to 224 (17 residues), 24.2 bits, see alignment (E = 9e-09) PF04879: Molybdop_Fe4S4" amino acids 238 to 289 (52 residues), 54.4 bits, see alignment 3.8e-18 TIGR01591: formate dehydrogenase, alpha subunit" amino acids 242 to 919 (678 residues), 920.6 bits, see alignment E=2.3e-281 PF00384: Molybdopterin" amino acids 294 to 724 (431 residues), 235 bits, see alignment E=5.3e-73 PF01568: Molydop_binding" amino acids 811 to 916 (106 residues), 89.6 bits, see alignment E=5.7e-29

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 98% identity to azl:AZL_a00170)

Predicted SEED Role

"NAD-dependent formate dehydrogenase alpha subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (951 amino acids)

>MPMX19_03821 Putative formate dehydrogenase (Azospirillum sp. SherDot2)
MTLIHETDYGTPARVSETLVTLEIDGRSISVPEGTSVMRAAMDAGITVPKLCATDSLEPF
GSCRLCAVEIEGRRGTPASCTTPVAPGMKVHTQTDKLAKLRRGVMELYISDHPLDCLTCA
ANGDCELQDMAGAVGLRNVRYGFEGENHRAAAKDESNPYFTFDASKCIVCSRCVRACQET
QGTFALTIDGRGFASSVSPGANESFMDSECVSCGACVQACPTATLTEKSIIEHGQPEHSV
ITTCAYCGVGCSFKAELQGTTVVRMTPWKDGGANEGHSCVKGRFAWGYATHKDRIMKPMV
REKITDPWREVSWEEAVAYAAERLKAVQAKYGRGSIGGITSSRCTNEEVYVVQKMIRAAF
GTNNIDTCARVCHSPTGYGLSQTFGTSAGTQDFRSVDKSDVILVIGANPTDGHPVFGSRM
KKRLRAGAKLIVVDPRRIDLVRSPHVQAEHHLQLQPGTNVAVVNAIAHVIVTEGLVNRSF
VEGRCDPKEFAKWEAFIAEHRNSPEYLESRTGVPADQVRAAARLYATGGNAAIYYGLGVT
EHSQGSSTVMAIANLAMATGNIGREGVGVNPLRGQNNVQGSCDMGSFPHELPGYRHVSDD
VVRQEFETAWGVTLDPEPGLRIPNMFDSAHDGSFRGLFVQGEDIVQSDPNTNHVTSALEA
LDIVIVQDLFLNETAAFAHVFFPGTSFLEKDGTFTNAERRINRVRKAMPSKVGRDEHWVA
CALATAMGYPMHYETTSEIMDEIARLTPTFRGVSFEKLDRVGSVQWPCTGFEDDDTGMAI
MHEESFARGLGRFVITEYVPTDERTTRMYPLVLTTGRILAHYNVGAQTRRTANVAWHPED
VLEINPVDAEIRGVKDGDMVSLASRMGATTLRAVISDRMPAGVVYTTFHHPVTGANVITT
ENSDWATNCPEYKVTAVQVARSNQPSDWQVDYAKNDVERKRIAAANVIAAE