Protein Info for MPMX19_03686 in Azospirillum sp. SherDot2

Annotation: Quinoprotein alcohol dehydrogenase (cytochrome c)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 638 signal peptide" amino acids 1 to 49 (49 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 48 to 602 (555 residues), 747.6 bits, see alignment E=3.7e-229 PF13360: PQQ_2" amino acids 96 to 266 (171 residues), 57.5 bits, see alignment E=2.5e-19 amino acids 516 to 584 (69 residues), 37.5 bits, see alignment E=3.1e-13 PF01011: PQQ" amino acids 117 to 142 (26 residues), 23.4 bits, see alignment (E = 5.7e-09) amino acids 166 to 200 (35 residues), 22.7 bits, see alignment (E = 9.2e-09) amino acids 548 to 584 (37 residues), 38 bits, see alignment 1.3e-13 PF13570: PQQ_3" amino acids 136 to 184 (49 residues), 20.8 bits, see alignment 6.4e-08 amino acids 526 to 566 (41 residues), 26.9 bits, see alignment 7.8e-10

Best Hits

Swiss-Prot: 69% identical to BOH_THABB: 1-butanol dehydrogenase (quinone) (boh) from Thauera butanivorans (strain ATCC 43655 / DSM 2080/ JCM 20651 / NBRC 103042 / IAM 12574 / Bu B1211)

KEGG orthology group: K00114, alcohol dehydrogenase (cytochrome c) [EC: 1.1.2.8] (inferred from 95% identity to azl:AZL_b04560)

MetaCyc: 69% identical to 1-butanol dehydrogenase (Thauera butanivorans)
RXN-17641 [EC: 1.1.5.11]

Predicted SEED Role

"Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8)" (EC 1.1.99.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.8, 1.1.99.8

Use Curated BLAST to search for 1.1.2.8 or 1.1.5.11 or 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (638 amino acids)

>MPMX19_03686 Quinoprotein alcohol dehydrogenase (cytochrome c) (Azospirillum sp. SherDot2)
MTLSSYSTIAAVTGGKPRRRFRGACLAGIAAVAAALFLAAAPAGPALASDVTWDAIANDA
KNPGDVLMYGMGVEGKRFSTLKQINTESVSRLVPAWSFSFGDEKQRGQETQALVHDGVIY
VTGSYSRMFALDAKTGKRLWSYSHRLPDDIRPCCDVVNRGAAIYGDKVFFGTLDASVVAL
NKDTGKVVWKKKFADHKVGYTMTGAPTIVKDQKTGKVLLIHGSSGDEFGVVGRLFARDPD
TGDEIWMRPLVEGHMGRLNGKDSTPTGSDKAPSWPRDKDGNLVEAWNHGGGAPWQSATFD
VETNTIVIGTGNPAPWNGWARWPGDSLYTSGQAYVDPTTGELKGFYQHTPNDTWDFSGNN
ELVLFDYTDASGKTVKATAHADRNGFFYVTDRTKLATAGGEANKPNSVLAAYPFVDGITW
AKGIDLKTGRPIENEGQRPPLPAEGEKRGKPVEVSPPFLGGKNWNPMSYSEDTKLFYIAA
NHWKEDYWTEHVGYNPGSAYLGMGFRIHKMFDDHVGSLRALDPKTGKIVWEHKEELPLWA
GTLATAGNLVFTGTGDGFVKAFDAKSGQELWHFQTGSGIVSSPITWEQDGVQYVGITTGY
GGAVPLWGGDLADLTTRVTQGGSFWVFKLADVKVSSSK