Protein Info for MPMX19_03563 in Azospirillum sp. SherDot2

Annotation: Propane 2-monooxygenase, reductase component

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00111: Fer2
  • amino acids 16 to 91 (76 residues), 53.3 bits, E=3.2e-18
• PF00970: FAD_binding_6
  • amino acids 118 to 209 (92 residues), 50.4 bits, E=3.7e-17
• PF00175: NAD_binding_1
  • amino acids 221 to 328 (108 residues), 70.6 bits, E=2.5e-23

Best Hits

Swiss-Prot: 38% identical to DMPP_PSEUF: Phenol hydroxylase P5 protein (dmpP) from Pseudomonas sp. (strain CF600)

KEGG orthology group: K00523, CDP-4-dehydro-6-deoxyglucose reductase [EC: 1.17.1.1] (inferred from 96% identity to azl:AZL_c02880)

MetaCyc: 38% identical to phenol hydroxylase reductase component (Pseudomonas sp. CF600)
RXN-17503 [EC: 1.14.13.244]

Predicted SEED Role

"Methane monooxygenase component C (EC 1.14.13.25)" (EC 1.14.13.25)

MetaCyc Pathways

• superpathway of C1 compounds oxidation to CO₂ (9/12 steps found)
• methane oxidation to methanol I (1/1 steps found)
• CDP-4-dehydro-3,6-dideoxy-D-glucose biosynthesis (2/3 steps found)
• nitrite-dependent anaerobic methane oxidation (2/3 steps found)
• benzene degradation II (aerobic) (1/3 steps found)
• superpathway of CDP-glucose-derived O-antigen building blocks biosynthesis (2/8 steps found)

KEGG Metabolic Maps

• Methane metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.14.13.25, 1.17.1.1

Use Curated BLAST to search for 1.14.13.244 or 1.14.13.25 or 1.17.1.1

Protein Sequence (352 amino acids)

>MPMX19_03563 Propane 2-monooxygenase, reductase component (Azospirillum sp. SherDot2)
MTAQALQNSPAVHTVRLEPVGIEMEVAEGETILDAAFRQGVSLMHGCKEGQCSACKCLLI
DGDVEMLKYSTFALSDPERDSNHILLCRSLAYSDVAVELLNYDEDLLSRSIPVKDFNARL
AAVEPLTHDIVAIALDLDQPMKFWAGQYVDITLPGIGLTRSFSMGNPPADGSRLEFIIKK
YPDGAFSRQLDGGLSVGDRVSVRGPYGSCFRREGREGPMILVGGGSGMAPLLSILRDQAA
SGETRPVRLFYGARSRKDLFHLDQFEEFARSLPDFAFIPALSHAEEGDGWTGETGFIHEV
LRRHLSGMDEVEEADVFSCGPPPMIDAVLPVLQMAGVDSARVYFDKFTPATR