Protein Info for MPMX19_03249 in Azospirillum sp. SherDot2
Annotation: Carnitine monooxygenase oxygenase subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00479, Rieske 2Fe-2S family protein (inferred from 64% identity to sit:TM1040_3219)MetaCyc: 59% identical to stachydrine N-demethylase oxygenase subunit (Sinorhizobium meliloti 1021)
R501-RXN [EC: 1.14.13.247]
Predicted SEED Role
"Benzoate 1,2-dioxygenase (EC 1.14.12.10)" (EC 1.14.12.10)
MetaCyc Pathways
- benzoate degradation I (aerobic) (1/2 steps found)
- proline betaine degradation I (1/3 steps found)
- meta cleavage pathway of aromatic compounds (4/10 steps found)
- toluene degradation IV (aerobic) (via catechol) (4/13 steps found)
- mandelate degradation to acetyl-CoA (7/18 steps found)
- superpathway of aerobic toluene degradation (11/30 steps found)
- superpathway of aromatic compound degradation via 3-oxoadipate (14/35 steps found)
- superpathway of aromatic compound degradation via 2-hydroxypentadienoate (10/42 steps found)
KEGG Metabolic Maps
- Benzoate degradation via CoA ligation
- Benzoate degradation via hydroxylation
- Fluorobenzoate degradation
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.14.12.10 or 1.14.13.247
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (411 amino acids)
>MPMX19_03249 Carnitine monooxygenase oxygenase subunit (Azospirillum sp. SherDot2) MLHDADILALLTARRPGYSLPRDLYTDPAAFRMDVDRIWYRRWLFAIPACEIPKAGNHVV HTVVDHTVIIVRGDDGAIRAFHNSCRHRGSVLCKGEKGSRPKIVCPYHQWTYGLDGSLVW ARDMGPEFDRRQHGLKPVHCREVAGLVYICLAETAPPFEEFAVEAARYLAPHDLVNSKVA HQSTIVEKGNWKLVWENNRECYHCAGNHPALCRTFPEDARLIGNGGNGLASELDAHRERC EAVGAPSEFRMAADGFWRFVRMPLLGSAESYTMDGKAAVARPNSSFPFRNAGALLKFLYP SSWNHFLGDHAIVFRVTPLGPTETEVTTKWLVHKDAVEGRDYDLNRLTEVWIATNAEDRT VVEDNQRGILSPSYEPGPLSNDHEAGIVQFQDWYAATLTGVPALHDAVAAE