Protein Info for MPMX19_02973 in Azospirillum sp. SherDot2

Annotation: 3-oxo-isoapionate-4-phosphate transcarboxylase/hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02788: RuBisCO_large_N
  • amino acids 18 to 129 (112 residues), 28.5 bits, E=1.6e-10
• PF00016: RuBisCO_large
  • amino acids 139 to 422 (284 residues), 255.6 bits, E=6.3e-80

Best Hits

Swiss-Prot: 48% identical to OIAT_XANP2: 3-oxo-isoapionate-4-phosphate transcarboxylase/hydrolase (oiaT) from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)

KEGG orthology group: K01601, ribulose-bisphosphate carboxylase large chain [EC: 4.1.1.39] (inferred from 76% identity to vap:Vapar_1945)

MetaCyc: 48% identical to 3-oxoisoapionate-4-phosphate transcarboxylase/hydrolase (Xanthobacter autotrophicus Py2)
RXN-20936 [EC: 3.7.1.28]

Predicted SEED Role

"similar to ribulose-1,5-bisphosphate carboxylase, Type III"

MetaCyc Pathways

• Rubisco shunt (10/10 steps found)
• Calvin-Benson-Bassham cycle (12/13 steps found)
• ethene biosynthesis V (engineered) (20/25 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (20/26 steps found)
• oxygenic photosynthesis (13/17 steps found)
• D-apionate degradation III (RLP transcarboxylase/hydrolase) (1/3 steps found)
• nucleoside and nucleotide degradation (archaea) (4/10 steps found)

KEGG Metabolic Maps

• Carbon fixation in photosynthetic organisms
• Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.39

Use Curated BLAST to search for 3.7.1.28 or 4.1.1.39

Protein Sequence (426 amino acids)

>MPMX19_02973 3-oxo-isoapionate-4-phosphate transcarboxylase/hydrolase (Azospirillum sp. SherDot2)
MHADRIEATYLIETPLAPGKVAEVMAGEQSCGTFTRVHGETDDLRARARAIVESVEKLDV
AETPALPNSWLERQRNPGPWQRARVTISFPTANIGANLPTLAATVAGNLYDLGEVTGLRL
ESLRLPQAYRAQFDLPRHGVAGTRRLTGVADGPLVGSIIKPNVGLSAEETGELVGTLCAA
GLDFIKDDEICADPADAPLAQRIPAVMDRVRRHQDRTGKHVMVAFNITDETDAMRRHADL
VEREGGACIMVSLNWCGYSAVQSLRRHSGLVLHGHRNGFGAFSRHPLLGIGFDAYQALWR
LAGVDHMHVHGLNGKFAQPDAEVIEGARACLAPLAADAPDASDAVMPAFSSGQWAGTVPV
TWEAVGTADLMFMSGGGIMAHPDGPAAGVQSIRQAWDAVRAGRTLDEAAAGQPELRRSLD
FFGKKG