Protein Info for MPMX19_02939 in Azospirillum sp. SherDot2

Annotation: Putative formate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 923 PF13510: Fer2_4" amino acids 5 to 81 (77 residues), 70 bits, see alignment E=6.4e-23 PF00111: Fer2" amino acids 8 to 62 (55 residues), 25.3 bits, see alignment 5.4e-09 PF13183: Fer4_8" amino acids 146 to 206 (61 residues), 30.4 bits, see alignment 2.2e-10 PF12838: Fer4_7" amino acids 150 to 207 (58 residues), 35.7 bits, see alignment 4.3e-12 PF00037: Fer4" amino acids 191 to 208 (18 residues), 25.5 bits, see alignment (E = 3.9e-09) PF04879: Molybdop_Fe4S4" amino acids 226 to 277 (52 residues), 59.3 bits, see alignment 1.3e-19 TIGR01591: formate dehydrogenase, alpha subunit" amino acids 231 to 913 (683 residues), 759.2 bits, see alignment E=1.6e-232 PF00384: Molybdopterin" amino acids 281 to 717 (437 residues), 230.2 bits, see alignment E=1.8e-71 PF01568: Molydop_binding" amino acids 804 to 910 (107 residues), 99.4 bits, see alignment E=5.6e-32

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 69% identity to oca:OCAR_6702)

MetaCyc: 68% identical to NAD-dependent formate dehydrogenase alpha subunit (Methylorubrum extorquens AM1)
1.2.1.2-RXN [EC: 1.17.1.9]

Predicted SEED Role

"tungsten-containing formate dehydrogenase alpha subunit" in subsystem Formate hydrogenase

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.1.9 or 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (923 amino acids)

>MPMX19_02939 Putative formate dehydrogenase (Azospirillum sp. SherDot2)
MSNGITFTLDGTEVEARNGETIWQVATRLGTEIPHLCHRPEPGYRPDGNCRACMVEIEGE
RVLAASCIRKPKPGMTVRTASERAKASRKLVFELLLADQPERESAHDPNSTFWNWADKVE
IAGSRFPAVDRPTKTDDMSHPAMAVQLDACIHCNLCVRACREVQVNDVIGMAARGHLEKI
VFDFDDPMGQSTCVACGECVQACPTGALMPATLVDEKGVYANAPDREVDSVCPYCGVGCQ
LTYKIKDNKIVAVDGKDGPSNQNRLCVKGRFGFDYAHHGDRLTVPLIRKEGVSKHDVDID
PMNPFTHFREASWDEALAVAAGGLRKIRDERGGNALAGFGSAKGSNEEAYLFQKLVRTGF
GTNNVDHCTRLCHASSVAALIEGVGSGAVSAPFMAAEDAEVIVIIGANPTENHPVAATFF
KNAAERGAKLIVMDPRGLAMARHATHMLQFTPGRDVSMLNAILHTIIAEGLYDRQYVQAN
TEDFEALAETVKDYPPEEMEKVCGIPAATLRTVARLFARSKASIIFWGMGVSQHIHGTDN
SRCLIALSLVTGQIGRPGTGLHPLRGQNNVQGASDAGLIPMFYPDYKSVEDPEVQSFYEN
YWGTKLDGKRGLTVVEIMDAIHDGRINGLYIEGENPAMSDPDVTHAREALAMLDHLVVQD
IFLTETAKYADVVLPASAWPEKDGTVINTNRQVQMGRAAVPLPGEARQDLWIIQDMARGL
GLNWNYTHVSEVFTEMVGAMPSLANISWERVEREGSVTYPVDSPDQPGHDIVFGNGFPTK
TGRGRFVPARPINPAETPDLDYPLVLTTGRQLEHWHTGSMTRRSSVLDAVEPEAVAYVSP
HDLTRAGMRTGDYMLVSSRRGTIRLKARIDDRMPVGLVFIPFAYAEAAANVLTNPQLDPF
GKIPEFKYCAIKIEKAQMAEAAE