Protein Info for MPMX19_02859 in Azospirillum sp. SherDot2
Annotation: L-asparaginase 2
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 61% identical to ASPG2_BACSU: L-asparaginase 2 (ansZ) from Bacillus subtilis (strain 168)
KEGG orthology group: K01424, L-asparaginase [EC: 3.5.1.1] (inferred from 97% identity to azl:AZL_b03010)MetaCyc: 56% identical to L-asparaginase 2 (Escherichia coli K-12 substr. MG1655)
Asparaginase. [EC: 3.5.1.1, 3.5.1.38, 3.5.5.4]
Predicted SEED Role
"L-asparaginase (EC 3.5.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 3.5.1.1)
MetaCyc Pathways
- L-citrulline biosynthesis (7/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- superpathway of L-citrulline metabolism (9/12 steps found)
- L-asparagine degradation I (1/1 steps found)
- L-glutamine degradation I (1/1 steps found)
- superpathway of L-aspartate and L-asparagine biosynthesis (3/4 steps found)
- L-asparagine degradation III (mammalian) (2/3 steps found)
- L-glutamate and L-glutamine biosynthesis (4/7 steps found)
- N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation (2/5 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Cyanoamino acid metabolism
- D-Glutamine and D-glutamate metabolism
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 3.5.1.1
Use Curated BLAST to search for 3.5.1.1 or 3.5.1.38 or 3.5.5.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (369 amino acids)
>MPMX19_02859 L-asparaginase 2 (Azospirillum sp. SherDot2) MFKTTTRTMGRSMGRKMAAAGLAALLLSGTATHAAFADDGLPNVTILATGGTIAGTGATS TTTVGYTAAKVGVEKLIEAVPELKKVATVKGEQVFQIASENMTNDHWLKLAKRVNELLAQ KDVDGIVITHGTDTIEETAYFLNLTVKSTKPVVIVGAMRPSTAISADGPVNLYNAVTLAG SKDAVGKGVLVSLNDQINAGRDVTKTNTSTADTFRTPELGFLGYMQDNKAHFYRQVVRKH TAEAEFDISNLDSLPQVDIVYGYANNNRTALDALVKAGAKGIVHAGVGDGSLSNAMKPGL IDARKQGVAIVRSSRVGNGIVARNGEANDDELDFVASDTLNPQKARILLMLALTRTTDSK AIQKMFYTY