Protein Info for MPMX19_02795 in Azospirillum sp. SherDot2
Annotation: Homoaconitase large subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01703, 3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit [EC: 4.2.1.33 4.2.1.35] (inferred from 36% identity to ttr:Tter_2762)Predicted SEED Role
"3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 4.2.1.33)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- L-leucine biosynthesis (6/6 steps found)
- 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
- L-isoleucine biosynthesis II (5/8 steps found)
- 2,5-xylenol and 3,5-xylenol degradation (1/13 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- C5-Branched dibasic acid metabolism
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.33, 4.2.1.35
Use Curated BLAST to search for 4.2.1.33 or 4.2.1.35
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (418 amino acids)
>MPMX19_02795 Homoaconitase large subunit (Azospirillum sp. SherDot2) MGYTITEKILARAAGLPAVRAGQDIKAKPDFVLAYDFPGYTDVYFKTMKEDFGIERVAEP ERFGIFIDHMVPTATAKEEELHINTRNWCAENDVPLFERHGIGHQVAAEVGYATPGAFVV HFDGHVSQLGTFGTLAIGLRRNVMEAFVSDTVSIRVPDTVRVNLRGSLRPGVMARDVFHH LVRRLGPSSCRFQVLELGGPSVAAMSTEGLQTITGLAMFTGALTAIVNPDPVRLDYALPR ARKRLEPVSSDPDAHYSAVHDIDLSDLEPIVVAPPTPADTRDLTEFLGLEINAGYLGSCA SGRLEDLRAAAKVLEGRRVKTGVSLHIVPTSQELMAAAAREGLVSTLVEAGAFVSSPSCD YCFGRIATMTAGQRAVSTGTLNVRGRMGSPDSEIYLCNAAVVAASAIEGRIADPRPYL