Protein Info for MPMX19_02215 in Azospirillum sp. SherDot2

Annotation: DNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 529 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details TIGR04120: DNA ligase, ATP-dependent, PP_1105 family" amino acids 3 to 527 (525 residues), 809 bits, see alignment E=1.1e-247 PF01068: DNA_ligase_A_M" amino acids 223 to 402 (180 residues), 102.9 bits, see alignment E=2e-33 PF04679: DNA_ligase_A_C" amino acids 423 to 512 (90 residues), 45.9 bits, see alignment E=6.1e-16

Best Hits

KEGG orthology group: K01971, DNA ligase (ATP) [EC: 6.5.1.1] (inferred from 93% identity to azl:AZL_003120)

Predicted SEED Role

"ATP-dependent DNA ligase (EC 6.5.1.1) LigC" in subsystem DNA Repair Base Excision (EC 6.5.1.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (529 amino acids)

>MPMX19_02215 DNA ligase (Azospirillum sp. SherDot2)
MNRFAALIDALVFMPSRNGKIRLLVEHFATAPDPERGWALAALTGALSFREAKPAAIREL
AATRVDPELLALSYDYVGDLAETVALIWPERPERANSLPPSLDEVVEALRAAKRGQVIGL
VEGWLDTLDSSGRFALLKLITGALRIGVSARLAKTALAEWGKAARPEVSVDDVEEVWHGL
NAPYVELFAWLEGRLGRPATGAGAGFRPMMLSHPLEEEDRAGLDPAAYAAEWKWDGIRVQ
MVARGGERRLYSRTGDDVSGAFPDIVDHMSFDAVLDGELLVARDGVVAPFNDLQQRLNRK
TVTAQMLRDGPAWVRLYDILFDGEEDLRGLSFVERRARLERWFEAVQPRRMDLSPLVPFD
RWDELVALREGARENGIEGLMLKRRDSAYLAGRPKGPWFKWKRGALTLDTVMMYAQRGHG
KRSSYYSDYTFGVWRGEELVPVGKAYSGFTDAELVELDRWVRNHTTRRYGPVREVEAGLV
LEVAFDSVHPSNRHKSGLAMRFPRIHRIRWDKPAHEADRLETLAAMVAG