Protein Info for MPMX19_02081 in Azospirillum sp. SherDot2

Annotation: Threonine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 654 PF02824: TGS" amino acids 9 to 68 (60 residues), 40.8 bits, see alignment 3.6e-14 TIGR00418: threonine--tRNA ligase" amino acids 79 to 638 (560 residues), 699.2 bits, see alignment E=2.5e-214 PF07973: tRNA_SAD" amino acids 176 to 226 (51 residues), 51.3 bits, see alignment 1.9e-17 PF00587: tRNA-synt_2b" amino acids 325 to 538 (214 residues), 176 bits, see alignment E=1.7e-55 PF03129: HGTP_anticodon" amino acids 550 to 639 (90 residues), 72.3 bits, see alignment E=6.2e-24

Best Hits

Swiss-Prot: 75% identical to SYT_RHOCS: Threonine--tRNA ligase (thrS) from Rhodospirillum centenum (strain ATCC 51521 / SW)

KEGG orthology group: K01868, threonyl-tRNA synthetase [EC: 6.1.1.3] (inferred from 97% identity to azl:AZL_024740)

Predicted SEED Role

"Threonyl-tRNA synthetase (EC 6.1.1.3)" (EC 6.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (654 amino acids)

>MPMX19_02081 Threonine--tRNA ligase (Azospirillum sp. SherDot2)
MVTSVTSNIAITLPDGSVREFDRPVTGLEIAQSIGSRLAKDALAVKIDGTVKDLTTTVTT
NAKIEIVTRNHGDALEVIRHDAAHVLADAVQKLYPGTQVTIGPSIATGFYYDFARDEPFT
PEDLEKIEAKMREIVGADIPIVREVWDRDEAVAYFKKLGEHYKAELIEAIPQGEPVSIYR
QGDWLDLCRGPHAMTTGKVGQGFKLMKVAGAYWRGDSRNPMLQRIYGTAWRDEKELKAYL
HQIEEAEKRDHRKLGKELDLFHVQEEAVGSVFWHPKGWTLYQTLENYIRTKLKSAGYVEV
KTPQLIDSSLFKASGHWDMYGDNMFKVEADGGEKLLGIKPMNCPGHVQIFRHGLRSYRDL
PIRMAEFGACHRNEPSGALHGIMRVRAFTQDDAHIFCTEDQVQSEAAEYFKLQLGVYKDL
GFDKISVKLALRPDVRTGADELWDRAEGALAQALRDAGLEYEELPGEGAFYGPKVEFHLT
DAIGRTWQCGTLQYDPNLPERLDASYIGEDGARHRPIMLHRAILGSMERFIGMLIEHYAG
KFPLWLSPVQVTVATITSEADGYAEEVAKLLKRKGLRVELDTRNEKINLKVREHSLQKVP
LMLVVGKREAEERTVALRVLGGKDQEILALDAAVAKLLEDAKSPAGDVVTDPPF