Protein Info for MPMX19_02000 in Azospirillum sp. SherDot2

Annotation: Aspartate-semialdehyde dehydrogenase 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01118: Semialdhyde_dh
  • amino acids 4 to 117 (114 residues), 113.6 bits, E=8e-37
• TIGR01296: aspartate-semialdehyde dehydrogenase
  • amino acids 5 to 334 (330 residues), 450.9 bits, E=1.4e-139
• PF02774: Semialdhyde_dhC
  • amino acids 140 to 319 (180 residues), 185.5 bits, E=1.1e-58

Best Hits

Swiss-Prot: 52% identical to DHAS_RICFE: Aspartate-semialdehyde dehydrogenase (asd) from Rickettsia felis (strain ATCC VR-1525 / URRWXCal2)

KEGG orthology group: K00133, aspartate-semialdehyde dehydrogenase [EC: 1.2.1.11] (inferred from 94% identity to azl:AZL_023980)

MetaCyc: 47% identical to aspartate semialdehyde dehydrogenase subunit (Bacillus subtilis)
Aspartate-semialdehyde dehydrogenase. [EC: 1.2.1.11]

Predicted SEED Role

"Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11)" in subsystem Lysine Biosynthesis DAP Pathway or Threonine and Homoserine Biosynthesis (EC 1.2.1.11)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• L-lysine biosynthesis I (9/9 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• superpathway of L-threonine biosynthesis (6/6 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (12/15 steps found)
• L-lysine biosynthesis III (6/7 steps found)
• L-lysine biosynthesis VI (6/7 steps found)
• L-homoserine biosynthesis (3/3 steps found)
• L-lysine biosynthesis II (7/9 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• dipicolinate biosynthesis (3/4 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• L-methionine biosynthesis IV (2/4 steps found)
• spermidine biosynthesis II (2/4 steps found)
• ectoine biosynthesis (2/5 steps found)
• 3-dehydroquinate biosynthesis II (archaea) (3/7 steps found)
• norspermidine biosynthesis (2/6 steps found)
• cremeomycin biosynthesis (2/7 steps found)
• grixazone biosynthesis (2/8 steps found)
• superpathway of polyamine biosynthesis III (2/8 steps found)
• platensimycin biosynthesis (6/26 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of plant hormones
• Glycine, serine and threonine metabolism
• Lysine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.11

Use Curated BLAST to search for 1.2.1.11

Protein Sequence (339 amino acids)

>MPMX19_02000 Aspartate-semialdehyde dehydrogenase 2 (Azospirillum sp. SherDot2)
MGYTVAVIGATGNVGREILQTLAERKFPADKVIALASEKSIGQEVSYGEDEILKVQDLNK
FDFHGVDIVLSSPGAKVSAIHVPRAAAAGAMVIDNTSQFRMDPDVPLVVPEVNPETLVGY
RKRGIIANPNGATIQMAVALKPLHDRFKIRRVVVSTYQSVSGAGKEAMDELFTQTRAIFV
NDPVAKSVFPKQIAFNVIPHIDAFMEDGSTREEWKMTVETKKILDPKIKVSATCVRVPTF
IGHALSINIETEEPISAEEARLVLRAAPGVSVIDLQTGDGYVTPVEIAGENPVFVSRIRD
DITVENGLSFWCVADNLRKGAALNAVQIAELLVRDYMVE