Protein Info for MPMX19_01630 in Azospirillum sp. SherDot2

Annotation: Phosphoribosylamine--glycine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 PF02844: GARS_N" amino acids 1 to 100 (100 residues), 130.5 bits, see alignment E=1.1e-41 TIGR00877: phosphoribosylamine--glycine ligase" amino acids 1 to 426 (426 residues), 537.8 bits, see alignment E=8.7e-166 PF01071: GARS_A" amino acids 101 to 297 (197 residues), 269.6 bits, see alignment E=5e-84 PF02786: CPSase_L_D2" amino acids 106 to 190 (85 residues), 26.1 bits, see alignment E=1.8e-09 PF02655: ATP-grasp_3" amino acids 117 to 212 (96 residues), 24.2 bits, see alignment E=9.5e-09 PF02843: GARS_C" amino acids 332 to 424 (93 residues), 105 bits, see alignment E=6.6e-34

Best Hits

Swiss-Prot: 63% identical to PUR2_BRUSU: Phosphoribosylamine--glycine ligase (purD) from Brucella suis biovar 1 (strain 1330)

KEGG orthology group: K01945, phosphoribosylamine--glycine ligase [EC: 6.3.4.13] (inferred from 95% identity to azl:AZL_019850)

MetaCyc: 57% identical to phosphoribosylamine--glycine ligase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylamine--glycine ligase. [EC: 6.3.4.13]

Predicted SEED Role

"Phosphoribosylamine--glycine ligase (EC 6.3.4.13)" in subsystem De Novo Purine Biosynthesis (EC 6.3.4.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (428 amino acids)

>MPMX19_01630 Phosphoribosylamine--glycine ligase (Azospirillum sp. SherDot2)
MKVLVVGSGGREHALCWAISNSPLCDALYCAPGNAGIADVATLVAIGSEDVDALVRFVQE
NAIDFVVVGPEGPLVLGLVDRLTALGVKAFGPSAAAAELEGSKGFMKDILAKYGVPTAFY
ERFKDPEAAKAYVRRHGAPIVVKADGLAAGKGVTVARTEQEAFEAIDDALVGGRFGAAGA
EVVVEEFLDGEEVSFFALCDGENALPLASAQDHKAVGDGDTGPNTGGMGAYSPAPVLTAD
LQDRVMREIILPTVKGMAAEGKPFKGVLFAGLMIMQTPDGPVPKTLEFNVRFGDPECQTL
MMRLKSDALAALVAAADGVLDSIDLRWHDRTALCVVMAANGYPGDYARNTEIRGLDKAGT
VDGVTVFHAGTRRAEDGRVLSVGGRVLGVTALAPTVAEAQTAAYKAVDALDWSGGFCRRD
IGWRAVGR