Protein Info for MPMX19_01627 in Azospirillum sp. SherDot2

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 443 PF01479: S4" amino acids 18 to 64 (47 residues), 40.8 bits, see alignment 1.5e-14 PF00849: PseudoU_synth_2" amino acids 82 to 210 (129 residues), 57.3 bits, see alignment E=2.3e-19 TIGR00093: pseudouridine synthase" amino acids 83 to 239 (157 residues), 130.5 bits, see alignment E=2.4e-42

Best Hits

KEGG orthology group: K06178, ribosomal large subunit pseudouridine synthase B [EC: 5.4.99.12] (inferred from 81% identity to azl:AZL_019810)

Predicted SEED Role

"tRNA pseudouridine synthase A (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (443 amino acids)

>MPMX19_01627 hypothetical protein (Azospirillum sp. SherDot2)
MDRSDTVKKSDSANNGGERIAKRLARAGLCSRRDAERWIAEGRVAVNSRVLDSPACVVRP
GDIVQVDGKVIPEPEPARLWRYHKPSGLVTTARDEKGRETVFDRLPPDLPRVVSIGRLDL
TTEGLLLLTNDGELARFLELPATGWTRRYRVRVFGEVDERQLAALEKGPTIEGVKYGPIE
AALDRIQGRNAWLTVSLKEGKNREIRKVMESLGLQVNRLIRVAYGPFQLGKLEEGAVEEV
PKRVVREQIASFFGTPEGAESTESGTKQRAKARAEAAGKSGPKAAAPSKGGAANADATKA
APKRAPRSATKRHEAEAARTEETKPARRGGATRGTLSLGGGSAKPGRSGKPEGARSDAPR
SEGARSRDDRARDDRAREDRPRDGKAPGFKPGGSKPTGPKGAGPKGAGPAPRGGPKAGGE
RSGDRPGGGAGGRTERPRADRRR