Protein Info for MPMX19_01545 in Azospirillum sp. SherDot2

Annotation: Lon protease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 804 PF02190: LON_substr_bdg" amino acids 10 to 200 (191 residues), 200.3 bits, see alignment E=8.5e-63 TIGR00763: endopeptidase La" amino acids 11 to 771 (761 residues), 1002.4 bits, see alignment E=9.3e-306 PF00004: AAA" amino acids 351 to 488 (138 residues), 80.4 bits, see alignment E=4.3e-26 PF07728: AAA_5" amino acids 351 to 483 (133 residues), 28.9 bits, see alignment E=2.6e-10 PF05362: Lon_C" amino acids 570 to 772 (203 residues), 327.6 bits, see alignment E=7.2e-102 PF13541: ChlI" amino acids 621 to 740 (120 residues), 34.1 bits, see alignment E=5.8e-12

Best Hits

Swiss-Prot: 92% identical to LON_AZOBR: Lon protease (lon) from Azospirillum brasilense

KEGG orthology group: K01338, ATP-dependent Lon protease [EC: 3.4.21.53] (inferred from 99% identity to azl:AZL_019140)

MetaCyc: 67% identical to Lon protease (Escherichia coli K-12 substr. MG1655)
Endopeptidase La. [EC: 3.4.21.53]

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type I" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (804 amino acids)

>MPMX19_01545 Lon protease (Azospirillum sp. SherDot2)
MIELSRGALYPVLPLRDIVVFPHMIVPLFVGREKSVRALEDVMKDDKQILLVTQKNAAQD
DPTPADIYSVGTVGTVLQLLKLPDGTVKVLVEGGQRAAITKFADNEEFFQAQAELVEEKT
GENQELEALSRAVVSQFEQYIKLNKKIPPEVLVSINQIEEAGKLADTVASHLALKIPEKQ
QLLECATVSERLERVYAFMEGEIGVLQVEKRIRNRVKRQMEKTQREYYLNEQLKAIQKEL
GETEDGRDESAELEEKINKTRFSKEAREKALAELKKLRSMSPMSAEATVVRNYLDWMLSI
PWKKRTKVKRDLKLAQKVLDDDHYGLEKVKERILEYLAVQNRMNKVKGPILCLVGPPGVG
KTSLGKSIGKATGRNFVRMSLGGVRDEAEVRGHRRTYIGSMPGKVIQGMKKAKSSNPLFL
LDEIDKLGADWRGDPSSALLEVLDPEQNGTFNDHYLEVDYDLSDVMFVCTANTMRMPQPL
LDRMEIIRVAGYTEDEKVEISKRHLIEKQIEANGLKKGEFTISDDALRDLVRYYTREAGV
RSLEREIANLCRKAVKEILLKSGGNAKVAVTRRNLDKYAGVRRFHYGEAELEDLVGVTTG
LAWTEVGGELLSIEAVGLPGKGRVTTTGKLGDVMKESVQAAESYVKSRAIAFGIKPTLFE
KKDIHVHVPEGATPKDGPSAGVAMATSIVSVLTGIPVRKDVAMTGEITLRGRVLPIGGLK
EKLLAALRGGIKHVLIPKDNEKDLADIPDNVKRGLDIMPVGTVDEVLRNALVRPLEPIEW
TEPEVDAAAAKAQEKGADGEALRH