Protein Info for MPMX19_01509 in Azospirillum sp. SherDot2

Annotation: DNA topoisomerase 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 934 PF01751: Toprim" amino acids 6 to 118 (113 residues), 75.1 bits, see alignment E=8e-25 TIGR01051: DNA topoisomerase I" amino acids 7 to 640 (634 residues), 789.9 bits, see alignment E=1.3e-241 PF01131: Topoisom_bac" amino acids 134 to 560 (427 residues), 423.6 bits, see alignment E=1.9e-130 PF01396: zf-C4_Topoisom" amino acids 607 to 641 (35 residues), 45.1 bits, see alignment (E = 1.4e-15) PF13368: Toprim_C_rpt" amino acids 661 to 747 (87 residues), 40.6 bits, see alignment E=4.2e-14 amino acids 752 to 803 (52 residues), 59.2 bits, see alignment 6.1e-20 amino acids 814 to 864 (51 residues), 74.3 bits, see alignment 1.2e-24

Best Hits

KEGG orthology group: K03168, DNA topoisomerase I [EC: 5.99.1.2] (inferred from 93% identity to azl:AZL_018530)

Predicted SEED Role

"DNA topoisomerase I (EC 5.99.1.2)" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA topoisomerases, Type I, ATP-independent or pVir Plasmid of Campylobacter (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (934 amino acids)

>MPMX19_01509 DNA topoisomerase 1 (Azospirillum sp. SherDot2)
MPGSNVVIVESPAKAKTINKYLGDDYTVIASFGHVRDLPARDGSVRPDEDFAMEWELGDR
SKRHIDEIAKAVKSADRIYLATDPDREGEAIAWHLSELLREKHLSDNRDVQRVTFNEITK
SAVQTAMANPRAVSQELVDAYLARRALDYLVGFTLSPVLWRKLPGSKSAGRVQSVALRLI
CERESEIEVFKPQEYWSIAVGFTTPAGSSFTASLTQLDGKRLDKFGLPNREVAEAAVARI
RPQAFAVSTVERKQSRRNPSPPFTTSTLQQEASRKLGFGATRTMRTAQKLYEGVDIGGET
VGLITYMRTDGVSLSQEAIDGARGLIGSHYGERYVPAQPRVYKTAAKNAQEAHEAIRPTD
LHRRPDSVAGYLGSDELRLYELIWKRTLASQMESAVLDQVAVDIASPSKDVVLRATGSIV
VFDGFLKVYQEDRDDAAEDDQQERRLPAMDQGDDLSRGDIDPEQHFTQPPPRYSEASLVK
KLEELGIGRPSTYASILQVLQDRNYVRLDKRRFIPEDRGRLVTAFLENFFHRYVEYNFTA
ELENQLDEISDGKIDWKTVLRDFWTAFDVAVNGTKDLTITQVLTTLDNELGAHFFPAATE
DGHDPRVCPVCREGRLGLKLGKMGAFIGCSRYPECRYTRPLAVANDENGEAQEGPRELGN
DPETGLPVTVRRGPYGAYIQLGPAPSAATPPVEEPPAEEPAADGKKPKAKKKKKDEAPKP
KRVSLPKGMAAADVDLDTALKLLALPRVIGNHPETAEEISAGIGRFGPYLKHGSVYKSLT
PDDDVLTIGINRAVDLLAGAAKKASAPAKTLGDHPKTGKPITMGSGRFGPYVKHASVYAS
IPKGTEPDSVTLEQALELIDAKVAKDAAKKGGKAAKDAEPAKAESAEAEKPAKAKAPAKT
AAKKPAAKKATKAKAAKDAPAEAAAPKKKTAKAS