Protein Info for MPMX19_01362 in Azospirillum sp. SherDot2

Annotation: putative lipid II flippase MurJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 524 signal peptide" amino acids 1 to 35 (35 residues), see Phobius details transmembrane" amino acids 50 to 70 (21 residues), see Phobius details amino acids 89 to 110 (22 residues), see Phobius details amino acids 133 to 153 (21 residues), see Phobius details amino acids 161 to 181 (21 residues), see Phobius details amino acids 189 to 216 (28 residues), see Phobius details amino acids 229 to 254 (26 residues), see Phobius details amino acids 274 to 292 (19 residues), see Phobius details amino acids 313 to 337 (25 residues), see Phobius details amino acids 349 to 370 (22 residues), see Phobius details amino acids 383 to 403 (21 residues), see Phobius details amino acids 409 to 428 (20 residues), see Phobius details amino acids 448 to 469 (22 residues), see Phobius details amino acids 478 to 502 (25 residues), see Phobius details TIGR01695: murein biosynthesis integral membrane protein MurJ" amino acids 4 to 500 (497 residues), 421.5 bits, see alignment E=2.5e-130 PF03023: MurJ" amino acids 29 to 467 (439 residues), 384.9 bits, see alignment E=6.9e-119 PF01554: MatE" amino acids 246 to 398 (153 residues), 33.6 bits, see alignment E=4.7e-12 PF14667: Polysacc_synt_C" amino acids 353 to 501 (149 residues), 45.9 bits, see alignment E=9.5e-16

Best Hits

Swiss-Prot: 41% identical to MURJ_RHITR: Probable lipid II flippase MurJ (murJ) from Rhizobium tropici

KEGG orthology group: K03980, virulence factor (inferred from 89% identity to azl:AZL_012660)

Predicted SEED Role

"Proposed peptidoglycan lipid II flippase MurJ" in subsystem ZZ gjo need homes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (524 amino acids)

>MPMX19_01362 putative lipid II flippase MurJ (Azospirillum sp. SherDot2)
MSFARAIATVGGLTLLSRLAGFARDILTAAVLGAGPVADAFFVALKLPNLFRRLFAEGAF
GVAFVPLFAAELQTRGRSAAIRFAEEALAMLLAMLLPFTLAAIVAMPWLMHGLAPGFVDE
PAKFALAVDMARLTFPYLALISLVALLGGVLNALDRFGPFAAAPIAFNLTLVAALLIAPR
LGLEPGTAMAAAVTLSGVVQVAWMAWACAKAGVVLTLRPPRMTEGMRRLFRLIGPGAIGA
GVMQINLFLNIVLASLLPSGAVSFLYYADRLNQMPLGVIGIAIGTALLPVLARHVAAGDE
RMVRHYLSRALEFSLLLGLPAAVALGVAGSPIVAVLFQRGAFGPEEAHATAMALAAYAIG
IPAYVIVKSLNAAFFARHDTVTPVRVAVIVTVATALLALLLMPWLGHVGIALSTGLTAWL
DVALLIAAMRKRGLFDLDERLKRRAPRIAAAAAGMGAAILAGGHVLTPWLDAPSTAVRFV
ALALLVAGGAAAFGALATLLGGARLGDVKGMLSKSAQPGKEQIG