Protein Info for MPMX19_01108 in Azospirillum sp. SherDot2

Annotation: Bifunctional enzyme IspD/IspF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 390 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF01128: IspD" amino acids 10 to 223 (214 residues), 230.2 bits, see alignment E=4.1e-72 TIGR00453: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase" amino acids 11 to 224 (214 residues), 231.7 bits, see alignment E=7.1e-73 PF12804: NTP_transf_3" amino acids 11 to 139 (129 residues), 47.3 bits, see alignment E=4.1e-16 TIGR00151: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase" amino acids 234 to 386 (153 residues), 208 bits, see alignment E=8.2e-66 PF02542: YgbB" amino acids 234 to 386 (153 residues), 217 bits, see alignment E=2.6e-68

Best Hits

Swiss-Prot: 63% identical to ISPDF_RHORT: Bifunctional enzyme IspD/IspF (ispDF) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K12506, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase / 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase [EC: 2.7.7.60 4.6.1.12] (inferred from 92% identity to azl:AZL_015110)

Predicted SEED Role

"2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60) / 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12)" in subsystem Isoprenoid Biosynthesis or polyprenyl synthesis or Teichoic and lipoteichoic acids biosynthesis (EC 2.7.7.60, EC 4.6.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.60 or 4.6.1.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (390 amino acids)

>MPMX19_01108 Bifunctional enzyme IspD/IspF (Azospirillum sp. SherDot2)
MPTCIAPTCIALIVAGGSGQRFGAERPKQYLDLAGKPVLRRTVEAFLGHPQVTGVRVVID
PAWRDAYDATLSGLALPDPVAGGASRQDSVRNGLEALATDGAPDLVLIHDAARPLIDADT
IAAVIAALDSTPGAIAAVPVADTLKRGSGDAITGTVDRDGLWRAQTPQGFRFPDILEAHR
AAMGLSLTDDAAVAERAGLTVALVPSKEDNFKVTTPDDLTRATRAIMSSLWDVRTGSGFD
VHRFTDGDFVMLCGLRVPHSHGLEGHSDADVGLHALTDAILGALAAGDIGSHFPPTDPRW
RGADSAKFLRHAADLVAERGGVIAHADVTVICERPKVGPHRAAMADRIAQILGIEVGRVS
VKATTTEQLGFTGRREGIAAQAVATVRLPG