Protein Info for MPMX19_00996 in Azospirillum sp. SherDot2

Annotation: Metalloprotease MmpA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 377 transmembrane" amino acids 6 to 27 (22 residues), see Phobius details amino acids 110 to 133 (24 residues), see Phobius details amino acids 292 to 313 (22 residues), see Phobius details amino acids 342 to 360 (19 residues), see Phobius details PF02163: Peptidase_M50" amino acids 13 to 354 (342 residues), 235.6 bits, see alignment E=8.9e-74 PF00595: PDZ" amino acids 144 to 187 (44 residues), 31.8 bits, see alignment 3e-11 TIGR00054: RIP metalloprotease RseP" amino acids 146 to 366 (221 residues), 164.4 bits, see alignment E=1.9e-52 PF17820: PDZ_6" amino acids 146 to 196 (51 residues), 46.6 bits, see alignment 5e-16 PF13180: PDZ_2" amino acids 147 to 204 (58 residues), 31.2 bits, see alignment E=4.4e-11

Best Hits

Swiss-Prot: 49% identical to Y1380_AGRFC: Putative zinc metalloprotease Atu1380 (Atu1380) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K11749, regulator of sigma E protease [EC: 3.4.24.-] (inferred from 96% identity to azl:AZL_016190)

Predicted SEED Role

"Membrane-associated zinc metalloprotease" in subsystem Sex pheromones in Enterococcus faecalis and other Firmicutes

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (377 amino acids)

>MPMX19_00996 Metalloprotease MmpA (Azospirillum sp. SherDot2)
MDVIGGFWTSVLAFLLVLTVLVFVHELGHYLVARRNGVRIETFSIGFGPEIFGFTDRAGT
RWKFSALPLGGYVKMFGDADPASTPGAHLTAMSAEERAVSFHHKRVGQRAAIVAAGPIAN
FVFSIVVLALLFMTAGQSFTPPDVGGIQPGSAAERAGIQPGDLILSVDGTGVQRFEEIRQ
IVSIRPGQPLAVELKRDGRVMTLTATPDSQAVTDRLGNAHQIGLLGISRGSVGMMRHDPL
TAVWQAGREVAGMITGTFTALGQMVQGSRGTEELGGPLRIAQMSGEVAQSGWYPLVWFMT
FLSVNLGLINLFPVPMLDGGHLLFYGIEKLLGRPLGARAQEYGFRIGLALVLTLMVFATW
NDLVQLRVVDFFRGLVS