Protein Info for MPMX19_00578 in Azospirillum sp. SherDot2

Annotation: Glutamate--cysteine ligase EgtA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 454 TIGR01436: glutamate--cysteine ligase" amino acids 11 to 454 (444 residues), 733.3 bits, see alignment E=3.8e-225 PF04107: GCS2" amino acids 51 to 358 (308 residues), 329.7 bits, see alignment E=8.8e-103

Best Hits

Swiss-Prot: 64% identical to GSH1_BRAJU: Glutamate--cysteine ligase, chloroplastic (GSH1) from Brassica juncea

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 96% identity to azl:AZL_007600)

MetaCyc: 64% identical to gamma-glutamylcysteine synthetase (Lotus japonicus)
Glutamate--cysteine ligase. [EC: 6.3.2.2]

Predicted SEED Role

"Glutamate--cysteine ligase (EC 6.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (454 amino acids)

>MPMX19_00578 Glutamate--cysteine ligase EgtA (Azospirillum sp. SherDot2)
MSAPPTTRGEPITDRRQLAAYLESGCKPAPDWRIGTEHEKFAYRTSDLRPLPYEGPDGIR
ELLTRMTRFGWQPVEEKGNIIALVQDMANITLEPGGQVELSGAPLETLHQTCAEVHRHLR
QVKEVGGELGIAMMGLGFQPKWTRDDIPWMPKGRYKIMRDYMPKVGSLGLDMMTRTCTVQ
VNLDFASEADMVKKFRVSLALQPIATALFAMSPFTEGKPNGFQSFRSHIWTDTDADRTGD
IPFVFEDGFGFERYVDYLLDTPMYFVYRDGTYIDAAGQSFRDFMDGKLPALPGELPLITD
WADHTTTAFPEARLKKYLEMRGADGGPWRSLCALPALWVGLLYDQVALDAAWDLVKDWTT
AERAHLRAEVPRSGLRTSFRTGTVRNVALEMLAIARDGLARRARNDNWGDDETHFLDTLQ
VVAETGRSPADELLEKFNGPWGGSVDPVFKEYAY