Protein Info for MPMX19_00549 in Azospirillum sp. SherDot2

Annotation: Formate dehydrogenase iron-sulfur subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 225 PF13247: Fer4_11" amino acids 52 to 164 (113 residues), 38.7 bits, see alignment E=3.4e-13 PF12797: Fer4_2" amino acids 79 to 99 (21 residues), 26.1 bits, see alignment (E = 2e-09) PF12837: Fer4_6" amino acids 79 to 102 (24 residues), 32.3 bits, see alignment (E = 2.3e-11) PF13237: Fer4_10" amino acids 81 to 123 (43 residues), 28.3 bits, see alignment 5e-10 PF00037: Fer4" amino acids 82 to 102 (21 residues), 32.4 bits, see alignment (E = 2e-11) PF12838: Fer4_7" amino acids 87 to 159 (73 residues), 31.3 bits, see alignment E=8.1e-11

Best Hits

Swiss-Prot: 64% identical to FDHB_WOLSU: Formate dehydrogenase iron-sulfur subunit (fdhB1) from Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC 11488 / FDC 602W)

KEGG orthology group: K00124, formate dehydrogenase, beta subunit [EC: 1.2.1.2] (inferred from 96% identity to azl:AZL_007320)

Predicted SEED Role

"Formate dehydrogenase-O, iron-sulfur subunit (EC 1.2.1.2); Putative formate dehydrogenase iron-sulfur subunit (EC 1.2.1.2)" (EC 1.2.1.2)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (225 amino acids)

>MPMX19_00549 Formate dehydrogenase iron-sulfur subunit (Azospirillum sp. SherDot2)
MARMKFLCDAERCIECNACVTACKNEHEIPWGINRRRVVTINDGKPGERTISVACMHCSD
APCKAVCPVDCFYQTDEGVVLHNKDLCIGCGYCFYACPFGAPQYPQAGNFGTRGKMDKCT
FCSGGPEPDNSEAEFKKYGRNRLAEGKLPLCAEMCSTKALLAGDADKVSDIYRERVAARG
FGSGAWGWGTAYQTRSGQERGQPGGSLFQGTGGPAGGGTLQKQGL