Protein Info for MPMX19_00470 in Azospirillum sp. SherDot2
Annotation: Cysteine desulfurase NifS
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 70% identical to NIFS_GLUDA: Cysteine desulfurase (nifS) from Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5)
KEGG orthology group: K04487, cysteine desulfurase [EC: 2.8.1.7] (inferred from 90% identity to azl:AZL_006550)MetaCyc: 41% identical to mitochondrial cysteine desulfurase NFS1 (Homo sapiens)
Cysteine desulfurase. [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]
Predicted SEED Role
"Cysteine desulfurase (EC 2.8.1.7), NifS subfamily" in subsystem Alanine biosynthesis or Nitrogen fixation (EC 2.8.1.7)
MetaCyc Pathways
- superpathway of thiamine diphosphate biosynthesis II (10/11 steps found)
- thiazole component of thiamine diphosphate biosynthesis II (6/7 steps found)
- superpathway of thiamine diphosphate biosynthesis I (8/10 steps found)
- L-alanine biosynthesis III (1/1 steps found)
- L-cysteine degradation IV (1/1 steps found)
- superpathway of L-alanine biosynthesis (3/4 steps found)
- thiazole component of thiamine diphosphate biosynthesis I (4/6 steps found)
- cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
- bis(guanylyl molybdopterin) cofactor sulfurylation (1/3 steps found)
- molybdopterin biosynthesis (3/6 steps found)
- tRNA-uridine 2-thiolation (thermophilic bacteria) (2/5 steps found)
- tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
- tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
- tRNA-uridine 2-thiolation (cytoplasmic) (1/8 steps found)
- [2Fe-2S] iron-sulfur cluster biosynthesis (2/10 steps found)
- tRNA-uridine 2-thiolation and selenation (bacteria) (2/11 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.8.1.7
Use Curated BLAST to search for 2.8.1.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (409 amino acids)
>MPMX19_00470 Cysteine desulfurase NifS (Azospirillum sp. SherDot2) MTIPNVTIPNRGVYLDNNATTRVDPDVLAEMLPLFTEHFGNPSSMHAFGAAVGGKIEWAR KQVQALLGAAHDSEIIFTSGGTESDNTAILSIIEAYPKKREIVTSVVEHPAVLALCDYLE KKRDYKIHRIPVDNKGNLDIDAYRAALSDQVAIVSIMWANNETGTIFPIEELAQMAKAVG AIFHTDAVQAVGKIPMKLADSAVDMLSLSGHKLHAPKGIGALYVKRGLRFRPMLRGGHQE RSRRAGTENAPAIVGLGAAAHLALTHMGEENTRVKAQRDRLEQAILAAVPACFVTGNPDN RLPNTCNIAFEYIEGEAILLLLNEQGIAASSGSACTSGSLEPSHVMRAMGVPYTAAHGAT RFSLSRDTTDAEIDHVIAVVPGIIAKLRSLSPYWQQEAGKPKEFAPVYS