Protein Info for MPMX19_00336 in Azospirillum sp. SherDot2

Annotation: Phosphomethylpyrimidine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 16 to 86 (71 residues), 82 bits, E=2.2e-27
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 101 to 549 (449 residues), 702.8 bits, E=7.1e-216
• PF01964: ThiC_Rad_SAM
  • amino acids 102 to 546 (445 residues), 624.6 bits, E=8e-192

Best Hits

Swiss-Prot: 80% identical to THIC_ZYMMO: Phosphomethylpyrimidine synthase (thiC) from Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 98% identity to azl:AZL_005290)

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (10/11 steps found)
• superpathway of thiamine diphosphate biosynthesis I (8/10 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Protein Sequence (575 amino acids)

>MPMX19_00336 Phosphomethylpyrimidine synthase (Azospirillum sp. SherDot2)
MPKDFNENAIRISTGPLPSSRKIHVPGTRFPDLRVALREIALSGGEPPLTVYDASGPYTD
ESVAIDVRAGLSKHRRDWILARGDVEPADNGVLRAKDGVAVTQMAYARAGIVTAEMEYIA
VRENLGRAAALEATRDGEDFGAEIPDFITPEFVRDEVARGRAIIPSNVNHPESEPMIIGR
NFLTKINANIGNSAVTSSIEEEVEKMVWSIRWGADTVMDLSTGADIHQTREWILRNSPVP
IGTVPIYQALEKVKGKAEDLTWELFRDTLIEQAEQGVDYFTIHAGLRLAHIPLTAKRVTG
IVSRGGSIIAKWCLAHHRENFLYDHFADIVEICRTYDVALSMGDGLRPGSQADANDAAQF
AELETLGELTKIAWARDVQVMIEGPGHVPMHKIKHNVEKQLALCGEAPFYTLGPLVTDIA
PGYDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLPDRDDVKAGVIAYKIAAHAADLAKGH
PAARLRDDAMSRARFSFRWLDQFALSLDPDTAMAYHDETLPAEGAKTAHFCSMCGPKFCS
MKITQEIRDAAESGMAEMSEKFRQSGGEIYQPAAE