Protein Info for MPMX19_00297 in Azospirillum sp. SherDot2

Annotation: 4-carboxy-4-hydroxy-2-oxoadipate aldolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03737: RraA-like
  • amino acids 24 to 172 (149 residues), 164.9 bits, E=7.9e-53

Best Hits

Swiss-Prot: 42% identical to HMGA_PSEP1: 4-hydroxy-4-methyl-2-oxoglutarate aldolase/4-carboxy-4-hydroxy-2-oxoadipate aldolase (Pput_1361) from Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)

KEGG orthology group: K10218, 4-hydroxy-4-methyl-2-oxoglutarate aldolase [EC: 4.1.3.17] (inferred from 92% identity to azl:AZL_004930)

MetaCyc: 43% identical to subunit of 4-hydroxy-4-methyl-2-oxoglutarate aldolase (Pseudomonas putida)
4-hydroxy-4-methyl-2-oxoglutarate aldolase. [EC: 4.1.3.17]; 4.1.3.17 [EC: 4.1.3.17]

Predicted SEED Role

"Dimethylmenaquinone methyltransferase"

MetaCyc Pathways

• 4-hydroxy-4-methyl-L-glutamate biosynthesis (1/2 steps found)
• gallate degradation I (2/4 steps found)
• methylgallate degradation (3/6 steps found)
• gallate degradation II (2/5 steps found)
• protocatechuate degradation I (meta-cleavage pathway) (4/8 steps found)
• superpathway of vanillin and vanillate degradation (5/10 steps found)
• syringate degradation (4/12 steps found)

KEGG Metabolic Maps

• Benzoate degradation via hydroxylation
• C5-Branched dibasic acid metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.3.17

Protein Sequence (222 amino acids)

>MPMX19_00297 4-carboxy-4-hydroxy-2-oxoadipate aldolase (Azospirillum sp. SherDot2)
MTVHKDFARPDAATLEALRAQSPATLHEAMGKKGAMGYPIKPLFPGMRLCGPALTVSCGP
TDNLMIHIAVALAKPGDVLVVDFKGMTDAGPWGDILTASAIARGLGGLVIDGCVRDAAAI
RDMGFPVFCRGTNMKGTNKTDAKGDVNTTVVVGGVMVSPGDIVVADDDGVVVVPQGLAAD
TLAKAAAREAAEEAYRHKLEAGETTIELLNLKPYLDAANIAL