Protein Info for MPMX19_00095 in Azospirillum sp. SherDot2

Annotation: ATP-dependent RNA helicase HrpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 854 TIGR01970: ATP-dependent helicase HrpB" amino acids 9 to 853 (845 residues), 1063.4 bits, see alignment E=0 PF00270: DEAD" amino acids 23 to 166 (144 residues), 34.5 bits, see alignment E=3.5e-12 PF00271: Helicase_C" amino acids 215 to 339 (125 residues), 45.5 bits, see alignment E=1.7e-15 PF04408: HA2_N" amino acids 401 to 429 (29 residues), 25.4 bits, see alignment (E = 2.3e-09) PF08482: HrpB_C" amino acids 709 to 840 (132 residues), 185.4 bits, see alignment E=1.2e-58

Best Hits

KEGG orthology group: K03579, ATP-dependent helicase HrpB [EC: 3.6.4.13] (inferred from 95% identity to azl:AZL_025880)

Predicted SEED Role

"ATP-dependent helicase HrpB"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (854 amino acids)

>MPMX19_00095 ATP-dependent RNA helicase HrpB (Azospirillum sp. SherDot2)
MSKPAFPALPIDPVLPALLEALDSRGTAVLQAPPGAGKTTRVPLALLEAGWLKGRKILVL
EPRRLAARAAARRMASMLGEAVGETVGYRVRLDTKVGPKTRIEVVTDGLFLRQLQEDPEL
PAVGAVLFDEFHERGIDSDLALALVQEARGALRDDLRLVVMSATLDAAPVAALLADAQGP
AAMVTSEGRAYPVETRHLDAPTQGTRIEDAVAAAVRRALREEPGNALVFLPGVGEIRRVQ
SLLDQSDLGPNTVIAPLYGDLSAEAQDRAIGPTPPGQRKIVLATSIAETSLTIEGIRIVV
DSGLMRVPRFDPRGGMTRLATVKVSQASAEQRRGRAGRLEPGVCYRLWPEATHKALAPFT
TPEIADADLAPLALELAVWGVSDPATLSWLDQPPAAAMAQARELLTGLGALDKSGAITPH
GRRMAGFGVHPRLAHMMLAGKAMGQGALACLVAALLGERDIVRSQPGFRDADLRLRVDLL
RGEERGGFGAARGLSVDRGGAQQAVKQARQWRRQLGVRDDDAMDSHAVGLLVALAYPDRI
GQRRPGGQPGGVAAQYRLSGGRGAYFQQAEPLSAEEWLAIADLDGAARESRIFLAAPVTL
ADLEESFADDIRTETIVAWDAREQMVLARRRRMLFALILKDEKLAKPPADAMTAAMIEGI
RALGPTCLPWTDELRKWRTRVMFLRAREGDVYDWPDLSDDALMDNLEEWLAPFLDGVSRR
AHLERIDLSSALRALLPWDLKTRLDAEAPTHVEVPSGSRIPIDYDGEEPVLAVRLQEMFG
LAETPRIAGGRVPMLLHLLSPARRPVQVTRDLASFWANAYKAVKADLKGQYPKHYWPDNP
LEAEPTARAKPRGR