Protein Info for MPMX19_00073 in Azospirillum sp. SherDot2

Annotation: Long-chain-fatty-acid--CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 35 to 413 (379 residues), 260 bits, E=3.3e-81
• PF13193: AMP-binding_C
  • amino acids 463 to 537 (75 residues), 82.2 bits, E=4.1e-27

Best Hits

KEGG orthology group: K00666, fatty-acyl-CoA synthase [EC: 6.2.1.-] (inferred from 95% identity to azl:AZL_026090)

Predicted SEED Role

"3-methylmercaptopropionyl-CoA ligase (DmdB)"

KEGG Metabolic Maps

• Alkaloid biosynthesis II
• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Caprolactam degradation
• Ethylbenzene degradation
• Geraniol degradation
• Limonene and pinene degradation
• Propanoate metabolism
• Tyrosine metabolism
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.-

Use Curated BLAST to search for 6.2.1.-

Protein Sequence (550 amino acids)

>MPMX19_00073 Long-chain-fatty-acid--CoA ligase (Azospirillum sp. SherDot2)
MSETAAAADVRANPYDRDLGRNAANFVALTPLTFLERAAAVWPDRPAVVHGTVRRTWAET
FIRVRRLAAALANVGIGTGDTVAMLAANTPELFEAHFGVPLAGAVLNAVNTRLDAEAIAF
ILKHGEAKILIVDREFSGVAKKALALLDAPIPVVDIDDPTYTGGELIGDRDYESFISDVG
AEHPWTLPADEWQAIALNYTSGTTGNPKGVVYHHRGAYLNAVSNALSWNMGDAPVYLWTL
PMFHCNGWCFPWTIAVTAGTAVCLRQVRPDTVLKLIRDERVTNFCGAPIVLNMLNNAPAE
LKQGIEQKVKVMVAGAAPPAAVIAGMERMGWEVTHVYGLTECYGPTVVCVWHDRWDGLSL
DEKAAIKARQGVRGPMLEAVIVADPFTLEPVPKDGRTMGEIMMRGNNVMKGYLKNPKATE
EAFTGGWFHTGDLAVWHEDGYVEIKDRSKDIIISGGENISSIEVEDVLYKHPDVLEAAVV
ARHDEKWGETPCAFVTLKDGAASTEADIIAFCRSHMAHFKCPRTVVFGPLPKTSTGKIQK
YVLRKQTEGL