Protein Info for MIT1002_04128 in Alteromonas macleodii MIT1002

Annotation: tRNA modification GTPase MnmE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 462 PF10396: TrmE_N" amino acids 10 to 124 (115 residues), 128.8 bits, see alignment E=4.3e-41 TIGR00450: tRNA modification GTPase TrmE" amino acids 15 to 462 (448 residues), 364.3 bits, see alignment E=1.1e-112 PF12631: MnmE_helical" amino acids 127 to 459 (333 residues), 211.6 bits, see alignment E=5.3e-66 TIGR00231: small GTP-binding protein domain" amino acids 220 to 376 (157 residues), 87.6 bits, see alignment E=7.8e-29 PF01926: MMR_HSR1" amino acids 222 to 340 (119 residues), 92.6 bits, see alignment E=6.6e-30 PF02421: FeoB_N" amino acids 222 to 378 (157 residues), 36.9 bits, see alignment E=9e-13 PF00071: Ras" amino acids 223 to 383 (161 residues), 25.2 bits, see alignment E=4e-09

Best Hits

Swiss-Prot: 67% identical to MNME_PHOLL: tRNA modification GTPase MnmE (mnmE) from Photorhabdus luminescens subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01)

KEGG orthology group: K03650, tRNA modification GTPase (inferred from 97% identity to amc:MADE_04098)

MetaCyc: 67% identical to 5-carboxymethylaminomethyluridine-tRNA synthase GTPase subunit (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"GTPase and tRNA-U34 5-formylation enzyme TrmE" in subsystem Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (462 amino acids)

>MIT1002_04128 tRNA modification GTPase MnmE (Alteromonas macleodii MIT1002)
MESLTLSTDTITAQATAPGRGGVGIVRVSGPKAKAIAEALVPSALTPRLATYTPFVDANN
SVIDQGIALFFKGPNSFTGEDVLELQGHGGQVVMDMLIDAVLATGDARLANPGEFSEQAF
LNDKLDLAQAEAIADLIDASSKQAAKSALRSLQGEFSTQIQTLSDQIVHLRMYVEAAIDF
PEEEIDFLSDGKVSGDLSAIMQSLTKVREQAKQGTLLREGMQVVIAGRPNAGKSSLLNAL
AGRDSAIVTDIAGTTRDVLKEHIHIDGMPVHIIDTAGLRESPDKVEQIGIERAWQAINEA
DHVLFVVDSTATVVIDPYEIWPEFMARLPQGIPVTVVRNKADLSTLDIGQSTVATEQGNI
SVINLSAKEGSGVDTLKAHLAKTMGFDTTTEGQFIARRRHIDALDQAYSYVSTGEQQLHD
AMAGELLAEELRLAHQALCEITGEFTSDDLLGKIFSSFCIGK