Protein Info for MIT1002_04101 in Alteromonas macleodii MIT1002

Annotation: Beta-glucosidase B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00232: Glyco_hydro_1
  • amino acids 13 to 443 (431 residues), 459.2 bits, E=6.6e-142
• TIGR03356: beta-galactosidase
  • amino acids 14 to 437 (424 residues), 580.1 bits, E=1.4e-178

Best Hits

KEGG orthology group: K05350, beta-glucosidase [EC: 3.2.1.21] (inferred from 70% identity to alt:ambt_22090)

Predicted SEED Role

"Beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism or Fructooligosaccharides(FOS) and Raffinose Utilization (EC 3.2.1.21)

MetaCyc Pathways

• cellulose degradation II (fungi) (2/3 steps found)
• neolinustatin bioactivation (2/3 steps found)
• linamarin degradation (1/2 steps found)
• lotaustralin degradation (1/2 steps found)
• linustatin bioactivation (2/4 steps found)
• coumarin biosynthesis (via 2-coumarate) (2/5 steps found)
• α-tomatine degradation (1/6 steps found)
• firefly bioluminescence (2/14 steps found)

KEGG Metabolic Maps

• Cyanoamino acid metabolism
• Phenylpropanoid biosynthesis
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.21

Use Curated BLAST to search for 3.2.1.21

Protein Sequence (445 amino acids)

>MIT1002_04101 Beta-glucosidase B (Alteromonas macleodii MIT1002)
MNSISLPAGSPLLKNSFLYGTATSSFQIEGDVDNRLECIWDRFCERPSAIADHSNGDVAC
DHIARWREDFDILLDLNVDAYRFSISWPRVITQEGELNPKGVAFYKTLVDALNEKGIKPF
VTLYHWDLPQHLEDKGGWLYRQTAFAFEGYVDKITQALGESVFSYATFNEPFCSAYLGYE
IGAHAPGRTSRKYGRTAAHHILLAHGLGMKVLRKNVPNSEAGIVLNFTPCYPATQKKEDL
LATRKADDYINQWYMQPVMEGSYPEVLFDIDEADRPPVEAGDMETICQPIDFLGVNFYTR
LHYSAPKEKGALYFEHPHQGPLTDIGWEIYPKALYDLLTSLNERYTLPPIFITENGAAMA
DELKDGLVNDVDRVDYYNAHLNMVHNAVIDGVEVSGYFAWSLLDNFEWAEGYEKRFGLVY
VDFETQKRTLKLSANAYKALIASRT