Protein Info for MIT1002_04087 in Alteromonas macleodii MIT1002

Annotation: Type-1 restriction enzyme R protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1093 PF04313: HSDR_N" amino acids 8 to 225 (218 residues), 150.4 bits, see alignment E=1.2e-47 TIGR00348: type I site-specific deoxyribonuclease, HsdR family" amino acids 72 to 739 (668 residues), 483.4 bits, see alignment E=6.2e-149 PF04851: ResIII" amino acids 313 to 484 (172 residues), 77.2 bits, see alignment E=3e-25 PF18766: SWI2_SNF2" amino acids 318 to 548 (231 residues), 258 bits, see alignment E=1.5e-80 PF22679: T1R_D3-like" amino acids 638 to 723 (86 residues), 87.5 bits, see alignment 2.7e-28

Best Hits

KEGG orthology group: K01153, type I restriction enzyme, R subunit [EC: 3.1.21.3] (inferred from 85% identity to ddc:Dd586_0400)

Predicted SEED Role

"Type I restriction-modification system, restriction subunit R (EC 3.1.21.3)" in subsystem Restriction-Modification System (EC 3.1.21.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.3

Use Curated BLAST to search for 3.1.21.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1093 amino acids)

>MIT1002_04087 Type-1 restriction enzyme R protein (Alteromonas macleodii MIT1002)
MTQFTPKFQEEYSAKLPALTLLTNLGWSFLSPELALTARGGKADEVVLHQVLRSELHKRT
FTFAGKSYPLSEKAIDNLIAEVCSPALNEGLLTANERLYNHLLYGISVTEFVDGKKASPT
VALIDWQNPTNNSFVFTEEFMVTRSGGVESRRPDIVCFVNGIPLVVIEAKRPDGNAKKGP
TIDEGISQSLRNQRHDEIPLLFAYSQMLLSINGNEGRYGTCGTPAKFWAAWREEDISDAE
MYALKNKTLKNEQIVGLFSHRPAKDLDWYKSLTAAGELAVTGQDQLLISLLSPTRLLDMT
RYFTLFDKKAGKIVARYQQVFGIKRLIERINTRSSTGGREGGVIWHTTGSGKSFTMVFLS
KALILHESLKQCRIVVVTDRVDLETQLSKTFASGGELAGKKDKEAAMATSGKRLAEQIGK
GTERIIFSLIQKFNTATKMPECVNLSSDIIVLIDEGHRSQGGENHIRMKQALPNAAFVAF
TGTPLLKDDKTTNKFGPIVHAYTMQRAVEDQTVTPLLYEERIPDLDVNERAIDSWFERIT
EGLSDEQKADLKRKFAKKGQVYGADDRIRLIALDIANHFIKNIDEGLKGQLACDSKVSAI
KYKKYLDEAGLFESAVVMSPPDSREGNTAVDEASTPEVTQWWKDNIGSQDEQAYTKHLIE
RFDKDDSLKILIVVDKLLTGFDEPKNTVLYIDKPLKEHNLIQAIARVNRLHPKKKFGLLI
DYRGILAELDTTIQKYQDLASRTQGGYDINDIAGLYNQMSTEYKRLPQLYKQLWAIFDGV
KNKNDIEQLRQVLVPKIEERGGELVDVHLKVREDFYEALTAFASCLKVALQSATFFEDKS
FNNQDRHHYKETVKQFSSLRQLVQQDAGERIDYDEYAEQVKKLLDKHVVGVEVKEPDGVY
EVGKMGQKQQPEDWSEDKTRNETDIIKTRVTRMIEQELRDDPYAQEAFSKLLRQAIEEAE
TLFDHPLKQYMLFREFEEQVQDRRLNDIPDAFAGNRHAQAYFGVFKKVLPEALAALDQQV
QDKWVKVAFEIDQAVETSVAENSINPQNIESDIRKKLLPRMFQECKVIGGGMDQAKKIVE
MIVQITRVGLNGD