Protein Info for MIT1002_03891 in Alteromonas macleodii MIT1002

Annotation: NADPH-dependent curcumin reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 332 PF16884: ADH_N_2" amino acids 5 to 113 (109 residues), 116.7 bits, see alignment E=6.8e-38 PF00107: ADH_zinc_N" amino acids 159 to 290 (132 residues), 78.8 bits, see alignment E=5.6e-26 PF13602: ADH_zinc_N_2" amino acids 191 to 330 (140 residues), 38.2 bits, see alignment E=4.1e-13

Best Hits

KEGG orthology group: K07119, (no description) (inferred from 92% identity to amc:MADE_03835)

Predicted SEED Role

"Quinone oxidoreductase (EC 1.6.5.5)" in subsystem ZZ gjo need homes (EC 1.6.5.5)

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.5

Use Curated BLAST to search for 1.6.5.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (332 amino acids)

>MIT1002_03891 NADPH-dependent curcumin reductase (Alteromonas macleodii MIT1002)
MSTYKAITLVNRPHDRKIGPHLFESKELEVPTPKDGQILIKQTHMSLDPAMLGWMSEDRE
SYIPPVELGDVMRSSGVGEVVESNNPNFSVGDKVMGMTGWSEYVLSDGSGFNKLQPGITE
EMALCVFALPGLTATTGLYNFGEPKEGETLIVTGAAGSVGSIVGQLAKADGLRVIGVVGS
EDKADWIVNELGFDGAINYKTDNLEEKLVELTPNKIDVFFENTGGPIQQHIFNRMNAHGR
IVVCGMISDYQSEEPSPGPNWIPLIKKRINIRGFAMPDHWGEIPQLLQKLSPYVQQGKIK
YRAHTVEGLENAEKGLNMLFEGKNTGKMIVKL