Protein Info for MIT1002_03840 in Alteromonas macleodii MIT1002

Annotation: Periplasmic beta-glucosidase precursor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF00933: Glyco_hydro_3" amino acids 70 to 393 (324 residues), 274.3 bits, see alignment E=2.5e-85 PF01915: Glyco_hydro_3_C" amino acids 434 to 641 (208 residues), 135.5 bits, see alignment E=3.7e-43 PF18559: Exop_C" amino acids 674 to 835 (162 residues), 158.6 bits, see alignment E=1.6e-50

Best Hits

KEGG orthology group: K01188, beta-glucosidase [EC: 3.2.1.21] (inferred from 91% identity to amc:MADE_03794)

Predicted SEED Role

"Periplasmic beta-glucosidase (EC 3.2.1.21)" in subsystem Beta-Glucoside Metabolism (EC 3.2.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.21

Use Curated BLAST to search for 3.2.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (850 amino acids)

>MIT1002_03840 Periplasmic beta-glucosidase precursor (Alteromonas macleodii MIT1002)
MRKSIIAKVVTGALVGVLVSACSAEKNTASVDDVEPQPNKAGEVWPKLDIEVKTNATVET
KVEEILSTMTTEQKIAQMIQPEIRNITVEDMRKYGFGSYLNGGGAFPNNDKHATPEDWVA
LAEKLYQASIDDSLDGSTIPTMWGTDAVHGHNNVIGATLFPHNIGLGAANNPELVEKIAH
ITAKEVMATGIDWVFAPTVAVVRDDRWGRTYESYSEDPAIVREYAASVVKGLQGAADKDF
LSDQRVISTVKHFVGDGGTVGGDDQGDNVASEQALFDIHAQGYVGGLTAGAQSVMASFNS
WNGEKLHGHKYLLTDVLKEQMGFDGFVVGDWNGHGQVKGCNNEDCAQAINAGLDIFMVPN
DWKVLYDNTLAQVNDGTIPMSRIDDAVRRILRVKVRAGLFEKPSPANRPLSGDRSLIGKA
EHREVAAQAVRESLVLLKNKNKTLPISASKRILVAGDGADNIGKQSGGWSITWQGTNNTN
DDFPGGSSIYDGIKAHVDNAGGNVQLSVDGSFETKPDVAIVVFGEEPYAEGHGDRETLIY
QHGSKTDLALLENLKSQGIPVVSVFISGRPMWVNAELNASDAFVAAWLPGSEGAAVADVL
FGEQDFKGKLSFSWPSEPQQIVNKGDETYEPLLPYGFGLTYSDDNVLSDTLNTEIKVSTD
SNKPMEILKGSVEAPWVLWLYSGDDSLAVNASTHTVGAITYRTVDKEVQEDARKITFDGT
EFAAMRFASKSFFREDLSAQLLTHGALSFNVSRHSDINAPVTVGMNCEGEGDAAGSCSTQ
IDISEQLKAMPADTWTNISIDLQCFVNEGIDFTGLVVPFELGSSGKASLSVADIQFTPEA
DKSASISCQN