Protein Info for MIT1002_03768 in Alteromonas macleodii MIT1002

Annotation: Putative DNA ligase-like protein/MT0965

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 561 TIGR04120: DNA ligase, ATP-dependent, PP_1105 family" amino acids 2 to 559 (558 residues), 676.2 bits, see alignment E=2.1e-207 PF01068: DNA_ligase_A_M" amino acids 203 to 434 (232 residues), 91 bits, see alignment E=8.5e-30 PF04679: DNA_ligase_A_C" amino acids 455 to 544 (90 residues), 45.4 bits, see alignment E=9e-16

Best Hits

Predicted SEED Role

"ATP-dependent DNA ligase (EC 6.5.1.1) LigC" in subsystem DNA Repair Base Excision (EC 6.5.1.1)

Isozymes

Compare fitness of predicted isozymes for: 6.5.1.1

Use Curated BLAST to search for 6.5.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (561 amino acids)

>MIT1002_03768 Putative DNA ligase-like protein/MT0965 (Alteromonas macleodii MIT1002)
MKAFSNLLEQLYYTSGNKAKAQLIADYIANTLDPDRGWAIAAMAGTLRFDFFKRNTVKKL
ITEHTDSALFAMSYDYVGEVSETVAHLWPYSEPTDNLPSLTEVVETFASVSKQKVSETLA
NYLTIMTPAQRWALLKLGTRGLRIGVSARSIKQILADYGNKDIKEIETLWHAVTPPYTDM
LAWLEGKADKPDIENAVTFHPVMLSHPIEDSDIDAFKENTWQIENKYDGIRVQLAVKSEP
IASKCATRKDQNDNKAGQTTNEDTPDKALFSRTGDDISHSFPDLLETVKGNMVLDGELLV
IHNVKGDAYDAMASQPNVDTFNALQQRLNKKKPSKALQASSPVGLIVYDALVLDGKDLTE
KTLKERRQALHEWFNHNKCERLFLSETLSASSPKSLRELHAHVCQNRAVEGLMIKRLDSK
YIPGRPKGQWFKWKRDPLVVDAVMMYAQRGHGKRSSFYSDYTFGAWEDDQLLPIGKAYSG
FTDEELKKLDNWVRRNAVGRFGPVREVKKDLVLEVAFDAVHPSSRHKSGVALRFPRIHRI
RWDKPAAEADTLTNVKALIEN