Protein Info for MIT1002_03756 in Alteromonas macleodii MIT1002

Annotation: Amylosucrase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 649 transmembrane" amino acids 347 to 364 (18 residues), see Phobius details amino acids 476 to 493 (18 residues), see Phobius details PF00128: Alpha-amylase" amino acids 114 to 317 (204 residues), 129 bits, see alignment E=3.2e-41 PF16657: Malt_amylase_C" amino acids 572 to 645 (74 residues), 28.8 bits, see alignment E=1.1e-10

Best Hits

KEGG orthology group: K05341, amylosucrase [EC: 2.4.1.4] (inferred from 88% identity to amc:MADE_00676)

Predicted SEED Role

"Sucrose phosphorylase (EC 2.4.1.7)" in subsystem Fructooligosaccharides(FOS) and Raffinose Utilization or Sucrose utilization or Sucrose utilization Shewanella (EC 2.4.1.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.7

Use Curated BLAST to search for 2.4.1.4 or 2.4.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (649 amino acids)

>MIT1002_03756 Amylosucrase (Alteromonas macleodii MIT1002)
MSYAADSGVCLDRILASTDLSGLSKKDATTFTRRLKEHFPRLFDKYTYVYGHQYDCYFHL
QKLVEILRDGLKSRKPALKKLDNERLKNPLWYRDEQQVGMACYVDLMGPTLNDLHDKIPY
FKSLGVTYVHLMPLYDAPKGDSDGGYAVSDYRKVNPTLGTVKDLEKLASALQKEGINLVL
DFVFNHTSDEHAWAKAALAGEKQFQDYYYLFDDRTIPDQYEHTLREIFPQVRRGSFTFNE
QIEKWVWTTFNSFQWDLNYSNPEVFNAITSEMLFLANIGSAALRLDALAFIWKELGTNCE
NQEKAHVLIQAFNCCLQIVAPAVVFKSEAIVHPDEVLKYIDKDECQLSYNPLLMALLWNS
LATRKTRLLTRSMKKSFSISENCTWVNYVRCHDDIGWTFDDAVANELGINAFDHRHFLNQ
FYTGRFEGSFANGVPFAENPSNGDCRVCGSLASLCGLEGALKSNDSKGIDDAVKRMMLLY
GITFSIGGIPLLYSSDEVGKLNDYSYRFDDTKKHDDRWVNRIAVTRADTELALGNTSPAN
PTETASLHVFNGLRKMIAIRKQNAIFGQSSTQILDADNHHCFAFLRENTQGHKLLVICNF
SEHPQTIQRSLLATLKGASSRDLLTDETVEMNASPLLLEPFQQMWLLAD