Protein Info for MIT1002_03723 in Alteromonas macleodii MIT1002

Annotation: L-lactate dehydrogenase [cytochrome]

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01070: FMN_dh
  • amino acids 23 to 362 (340 residues), 405.7 bits, E=8.3e-126

Best Hits

KEGG orthology group: K00104, glycolate oxidase [EC: 1.1.3.15] (inferred from 51% identity to azo:azo0609)

Predicted SEED Role

"L-lactate dehydrogenase (EC 1.1.2.3)" in subsystem L-rhamnose utilization or Lactate utilization or Respiratory dehydrogenases 1 (EC 1.1.2.3)

MetaCyc Pathways

• photorespiration I (7/9 steps found)
• methylglyoxal degradation V (2/3 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (4/8 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.3

Use Curated BLAST to search for 1.1.2.3 or 1.1.3.15

Protein Sequence (365 amino acids)

>MIT1002_03723 L-lactate dehydrogenase [cytochrome] (Alteromonas macleodii MIT1002)
MLKTPLNTIPKTLAALSDYENLARERLTPQSWAYIAGGSADELSMRRNREKLDDLCLLPR
ALKDVKLGGTKTTLLGQQFDHPIISGPVAYQALAHPDGEIATAMATQAQGGLWVMSTLAS
CSFEDISNQVESPRWFQLYVQPTRSQTLELIQKAEHFQFSALVITIDAPINGLRNREQRA
EFSLPPNVRAVNIDTPPPLVHPREGKSVVFQGLMAQAPTWDDIAFIQQHTSLPIVLKGIL
NPLDAQKAAELGVAGIVVSNHGGRALDSVPSPVEMLPIIRQTVGDEMMVLADSGVRRGAD
VVKLIALGANAVLIGRPLMYGLATAGALGVAHTIRLLRDELEMTMALCGVGTIGEINEHC
LWPKD