Protein Info for MIT1002_03558 in Alteromonas macleodii MIT1002

Annotation: dTDP-4-dehydrorhamnose reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 289 PF04321: RmlD_sub_bind" amino acids 1 to 287 (287 residues), 314.8 bits, see alignment E=1.2e-97 TIGR01214: dTDP-4-dehydrorhamnose reductase" amino acids 3 to 286 (284 residues), 308.9 bits, see alignment E=1.4e-96 PF02719: Polysacc_synt_2" amino acids 3 to 104 (102 residues), 23.3 bits, see alignment E=8.2e-09 PF01370: Epimerase" amino acids 3 to 171 (169 residues), 59.6 bits, see alignment E=8e-20 PF16363: GDP_Man_Dehyd" amino acids 34 to 135 (102 residues), 29.9 bits, see alignment E=1.1e-10 PF07993: NAD_binding_4" amino acids 52 to 154 (103 residues), 24.8 bits, see alignment E=2.8e-09

Best Hits

Swiss-Prot: 40% identical to RMLD_AGGAC: dTDP-4-dehydrorhamnose reductase (rmlD) from Aggregatibacter actinomycetemcomitans

KEGG orthology group: K00067, dTDP-4-dehydrorhamnose reductase [EC: 1.1.1.133] (inferred from 80% identity to amc:MADE_00990)

MetaCyc: 40% identical to dTDP-6-deoxy-L-talose 4-dehydrogenase (NAD+) (Aggregatibacter actinomycetemcomitans)
RXN-13749 [EC: 1.1.1.339]

Predicted SEED Role

"dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133)" in subsystem Rhamnose containing glycans or dTDP-rhamnose synthesis or linker unit-arabinogalactan synthesis (EC 1.1.1.133)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.133 or 1.1.1.339

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (289 amino acids)

>MIT1002_03558 dTDP-4-dehydrorhamnose reductase (Alteromonas macleodii MIT1002)
MNIVIIGKSGQLAFELSRELENSEHQVSFLGRDDIDITSASNIEETLSPLSAEVVINASA
YTAVDKAEEDTEACNAINTLAVKNLANFCKASGAFMVHVSTDYVFNGHKGSPYLTDDTIE
PQGAYGKSKAKGEKALLEILPDASCLIRTAWVYSSHGNNFVKTMLRLMADKPQLGVIDDQ
IGTPTWAKGLAEVCVTAALNKTSGVYHWTDEGVASWYDFALAIQELGVEKGLLDKSVPVL
PIPSSQYPTPAKRPHYSVLDKQTAREAFAACQPTHWRKQLASMLDELKA